BioVector? NTCC? FGY217釀酒酵母（Saccharomyces cerevisiae）PEP4基因缺失單倍體菌株

BioVector? FGY217菌株是釀酒酵母（Saccharomyces cerevisiae）的一種單倍體菌株，常用于分子生物學(xué)中的蛋白質(zhì)表達(dá)研究。它特別值得注意，因為該菌株缺失了PEP4基因，因此可用于過量表達(dá)那些通常會被液泡中的蛋白酶降解的蛋白質(zhì)。
遺傳背景
根據(jù)多個來源，F(xiàn)GY217菌株的具體基因型為MATa, ura3-52, lys2Δ201, pep4Δ。
MATa：指定酵母的交配型。
ura3-52：一個突變，使該酵母菌株對尿嘧啶呈營養(yǎng)缺陷型，這使得研究人員能夠利用尿嘧啶缺乏作為選擇標(biāo)記。
lys2Δ201：lys2基因的一個突變，導(dǎo)致賴氨酸缺陷，也可用于篩選。
pep4Δ：PEP4基因的一個缺失突變。該基因編碼一種液泡內(nèi)肽酶。它的缺失會使這種蛋白酶失活，并且重要的是，還會導(dǎo)致其他液泡水解酶的總體水平降低，從而防止不必要的蛋白質(zhì)降解。
研究應(yīng)用
FGY217菌株在以下應(yīng)用中非常有價值：
膜蛋白表達(dá)：它常用于異源表達(dá)真核膜蛋白，例如鉀離子通道，否則這些蛋白質(zhì)可能難以大量生產(chǎn)。
優(yōu)化研究：該菌株常用于高通量優(yōu)化方案中，以生產(chǎn)蛋白質(zhì)。研究表明，與其他菌株相比，F(xiàn)GY217中的蛋白質(zhì)表達(dá)水平通常更高。
GFP融合研究：它與生產(chǎn)綠色熒光蛋白（GFP）融合蛋白的系統(tǒng)兼容，使研究人員能夠追蹤蛋白質(zhì)定位并監(jiān)測表達(dá)水平。
過表達(dá)篩選：FGY217可用于通過同源重組插入基因，從而快速篩選和評估各種蛋白質(zhì)的過表達(dá)潛力。

The BioVector? FGY217 strain is a haploid yeast strain of Saccharomyces cerevisiae, commonly used in molecular biology for protein expression studies. It is particularly notable because it has a deletion of the PEP4 gene, which makes it useful for overexpressing proteins that would normally be degraded by proteases in the vacuole.
Genetic background
According to multiple sources, the specific genetic makeup of the FGY217 strain is MATa, ura3-52, lys2Δ201, pep4Δ.
MATa: Specifies the mating type of the yeast.
ura3-52: A mutation that makes the yeast strain auxotrophic for uracil, allowing researchers to use uracil deficiency as a selection marker.
lys2Δ201: A mutation in the lys2 gene causing a lysine deficiency, which can also be used for selection.
pep4Δ: A deletion mutation in the PEP4 gene. This gene encodes a vacuolar endopeptidase. Its deletion inactivates this protease and, importantly, leads to the overall reduction of other vacuolar hydrolases, which prevents unwanted protein degradation.
Research applications
The FGY217 strain is highly valued for the following applications:
Membrane protein expression: It is frequently used for the heterologous expression of eukaryotic membrane proteins, such as potassium channels, which might otherwise be difficult to produce in large quantities.
Optimization studies: The strain is often used in high-throughput optimization schemes for producing proteins. Studies have shown that protein expression levels are often higher in FGY217 compared to other strains.
GFP-fusion studies: It is compatible with systems for producing green fluorescent protein (GFP)-fusion proteins, allowing researchers to track protein localization and monitor expression levels.
Overexpression screening: FGY217 is used for rapidly screening and evaluating the potential for overexpression of various proteins by inserting genes using homologous recombination.

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