BioVector? NTCC? CHON-001永生化的人類軟骨細胞系immortalized human chondrocyte cell line BioVector NTCC典型培養(yǎng)物保藏中心

The NTCC? CHON-001 cell line is an immortalized human chondrocyte cell line developed for use in research related to cartilage biology and disease, particularly osteoarthritis.
Key characteristics
Origin: Isolated in 2001 from the cartilage of an 18-week-old female human fetus.
Immortalization: The primary chondrocytes were immortalized using a retrovirus containing the human telomerase reverse transcriptase (hTERT) gene.
Morphology: The cells exhibit a fibroblast-like morphology.
Karyology: It is a diploid cell line with a stable karyology. It has a modal chromosome number of 46 in 93% of the cells examined.
Culture: CHON-001 cells are typically cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS) and other additives. They can also be cultured in 3D structures like alginate beads to promote a more chondrocyte-like phenotype.
Response to stimuli: The cell line is responsive to inflammatory stimuli like interleukin-1β (IL-1β), making it a valuable in vitro model for studying osteoarthritis.
Applications
Osteoarthritis (OA) research: CHON-001 is used to study the mechanisms of inflammation and cartilage degradation involved in OA.
Drug development: The cell line serves as a tool for testing potential therapeutic agents for cartilage repair and OA treatment.
Cartilage biology: It is used to investigate chondrocyte signaling pathways, gene expression, and matrix synthesis.
Chondrogenesis: Used to study the process of cartilage formation and maturation.

CHON-001細胞系是一種永生化的人類軟骨細胞系，專門用于軟骨生物學和疾病（尤其是骨關(guān)節(jié)炎）相關(guān)的研究。
主要特征
來源：2001年，研究人員從一名18周大女性人類胎兒的軟骨中分離并建立了該細胞系。
永生化：利用含有端粒酶逆轉(zhuǎn)錄酶（hTERT）基因的逆轉(zhuǎn)錄病毒，將原代軟骨細胞永生化。
形態(tài)：細胞呈現(xiàn)成纖維細胞樣形態(tài)。
染色體核型：這是一個穩(wěn)定的二倍體細胞系。經(jīng)檢查，93%的細胞染色體眾數(shù)為46條。
培養(yǎng)：CHON-001細胞通常在添加了胎牛血清（FBS）和其他添加劑的杜氏改良伊格爾培養(yǎng)基（DMEM）中培養(yǎng)。為了促進更具軟骨細胞的表型，也可以在海藻酸鈉凝膠珠等三維結(jié)構(gòu)中培養(yǎng)。
對刺激的反應(yīng)：該細胞系對白細胞介素-1β（IL-1β）等炎癥刺激有反應(yīng)，使其成為研究骨關(guān)節(jié)炎的寶貴體外模型。
應(yīng)用
骨關(guān)節(jié)炎（OA）研究：CHON-001細胞被用于研究與OA相關(guān)的炎癥和軟骨退化機制。
藥物開發(fā)：該細胞系是測試用于軟骨修復(fù)和OA治療的潛在藥物的工具。
軟骨生物學：用于研究軟骨細胞的信號通路、基因表達和基質(zhì)合成。
軟骨形成：用于研究軟骨形成和成熟的過程。

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
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