BioVector? NTCC? pTM1_VP30_ZEBOV扎伊爾埃博拉病毒VP30表達(dá)質(zhì)粒BioVector?載體

pTM1_VP30_ZEBOV
(Plasmid BioVector?-069119)
Purpose
Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter
Backbone
Vector backbonepTM1
Backbone manufacturerBernhard Moss, National Institutes of Health
Backbone size w/o insert (bp)5357
Total vector size (bp)7136
Vector typeT7
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberUnknown
Gene/Insert
Gene/Insert nameVP30 ZEBOV
Insert Size (bp)866
Entrez GeneVP30 (a.k.a. ZEBOVgp5)
PromoterT7
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning sitePstI (not destroyed)
5′ sequencing primerATT GTA TGG GAT CTG ATC TGG
3′ sequencing primerGCC AAC TCA GCT TCC TTT CGG

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
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