BioVector? NTCC? AAVS1-tdTomato人AAVS1靶向熒光表達質(zhì)粒載體BioVector? plasmid vector

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Backbone
Vector backbonepUC57
Vector typeMammalian Expression, AAV ; S1 knockin donor vector
Selectable markersPuromycin
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert
Gene/Insert nametdTomato
Insert Size (bp)1431
PromoterCAGGS
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning sitePacI (not destroyed)
5′ sequencing primerggcggctctagagcctctgctaacc
3′ sequencing primerttggcagagggaaaaagatctcagtgg

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com