BioVector? NTCC? A19 E.coli strain BioVector?大腸桿菌菌株

特性 詳情
生物體 大腸桿菌 K-12 (Migula) Castellani and Chalmers
菌株名稱 A19 NTCC?
形態(tài) 革蘭氏陰性、桿狀細菌
生長方式 肉湯或瓊脂培養(yǎng)（好氧條件）
核型 Hfr（高頻重組）
基因型注釋 his 標記（可能回復）；酵母提取物可滿足的未識別生長要求；內切核酸酶 I (endA) 和 RecBCD 缺陷
生物安全等級 BSL 1 級（非致病性實驗室菌株）
登錄號 CGSC #7376；BGSC ECE 19
培養(yǎng)條件
培養(yǎng)基：
推薦：Luria-Bertani (LB) 液體培養(yǎng)基或 LB 瓊脂平板。在特定實驗方案中也會使用 2x YT 或 Tryptic Soy Broth (TSB) 等其他培養(yǎng)基。
最佳溫度：37°C。
氣氛：好氧條件。
生長速度：快速；在有利條件下，繁殖時間短至 20 分鐘。
應用
A19 菌株是幾個研究領域的基礎工具：
無細胞蛋白合成 (CFPS)：主要應用是生產 A19 細胞的 S30 提取物。這些提取物用于體外轉錄和翻譯系統(tǒng)，在沒有完整活細胞的情況下生產蛋白質，這對于合成膜蛋白或難以在體內表達的蛋白質特別有用。
遺傳學研究：由于其 Hfr 狀態(tài)和充分了解的基因組，它被用作研究細菌遺傳學、基因調控和 DNA 修復機制的模型生物。
生物技術/蛋白質生產：作為重組 DNA 技術和酶及其他蛋白質高產生產的標準宿主。
誘變研究：已用于分離對某些抗生素（如鏈佐絲菌素）具有抗性的突變體的研究，以了解耐藥機制。
General Information
Property Detail
Organism Escherichia coli K-12 (Migula) Castellani and Chalmers
Strain Designation BioVector? A19
Morphology Gram-negative, rod-shaped bacterium
Growth Mode Broth or agar culture (aerobic conditions)
Karyotype Hfr (High Frequency of Recombination)
Genotype Notes his marker (may revert); an unidentified growth requirement satisfied by yeast extract; deficient in Endonuclease I (endA) and RecBCD
Biosafety Level BSL 1 (Non-pathogenic laboratory strain)
Accession Numbers CGSC #7376; BGSC ECE 19
Culture Conditions
Culture Medium:
Recommended: Luria-Bertani (LB) liquid medium or LB agar plates. Other media like 2x YT or Tryptic Soy Broth (TSB) are also used in specific protocols.
Optimal Temperature: 37°C.
Atmosphere: Aerobic conditions.
Growth Rate: Rapid; takes as little as 20 minutes to reproduce under favorable conditions.
Applications
The A19 strain is a foundational tool in several research areas:
Cell-Free Protein Synthesis (CFPS): A major application is the production of S30 extracts from A19 cells. These extracts are used in in vitro transcription and translation systems to produce proteins without intact living cells, which is particularly useful for synthesizing membrane proteins or proteins that are difficult to express in vivo.
Genetics Research: Due to its Hfr status and well-understood genome, it is used as a model organism to study bacterial genetics, gene regulation, and DNA repair mechanisms.
Biotechnology/Protein Production: Serves as a standard host for recombinant DNA technology and the high-yield production of enzymes and other proteins.
Mutagenesis Studies: Has been used in studies to isolate mutants with resistance to certain antibiotics like streptothricin to understand drug resistance mechanisms.

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