主要特點

• 來源： 源自正常人新生兒包皮角質(zhì)形成細胞，通過表達人端粒酶逆轉(zhuǎn)錄酶（hTERT）基因?qū)崿F(xiàn)永生化。

• 分化能力： 該細胞系保留了分化能力，可以在3D類器官細胞培養(yǎng)模型中形成完全分層的、功能性的表皮（稱為人表皮等效物，HEEs），形態(tài)上非常接近正常的成人皮膚。

• 核型： N/TERT-1細胞系為二倍體，并多一條20號染色體（47, XY, +20）。

• 應(yīng)用： 它是研究表皮生物學(xué)、炎癥性皮膚?。ㄈ玢y屑病和特應(yīng)性皮炎）的發(fā)病機制、毒理學(xué)以及藥物篩選的重要工具。

• 減少變異性： 與原代角質(zhì)形成細胞不同，原代細胞存在顯著的供體間差異，而N/TERT-1細胞提供了穩(wěn)定且無限的細胞來源，這對于大規(guī)模和可重復(fù)的研究至關(guān)重要。

• 易于基因操作： 該細胞系易于進行基因編輯技術(shù)（如CRISPR-Cas9）操作，使研究人員能夠研究特定基因在皮膚生物學(xué)和疾病中的功能。

• 優(yōu)于HaCaT細胞系： 研究表明，在形成生物學(xué)相關(guān)模型方面，N/TERT-1細胞優(yōu)于廣泛使用的HaCaT細胞系，尤其是在分化和對炎癥刺激的反應(yīng)方面。

NTCC? N/TERT-1 cell line is an immortalized human keratinocyte cell line widely used in dermatology and skin biology research as a stable and reproducible alternative to primary human keratinocytes.

• Origin: Derived from normal human neonatal foreskin keratinocytes that were immortalized by the expression of the human telomerase reverse-transcriptase (hTERT) gene.

• Differentiation: The cells retain the ability to differentiate and form a fully stratified, functional epidermis (known as human epidermal equivalents, or HEEs) in 3D organotypic cell culture models, closely mimicking normal human skin morphology and barrier properties.

• Karyotype: The N/TERT-1 cell line is diploid with an additional chromosome 20 (47, XY, +20).

• Usage: It is a valuable tool for studying epidermal biology, the pathogenesis of inflammatory skin diseases (such as psoriasis and atopic dermatitis), toxicology, and for drug screening purposes.

• Reduced Variability: Unlike primary keratinocytes, which show significant donor-to-donor variability, N/TERT-1 cells provide a consistent and unlimited cell source, which is crucial for large-scale and reproducible research.

• Genetic Amenability: The cell line is amenable to genetic manipulation techniques like CRISPR-Cas9, allowing researchers to study the function of specific genes in skin biology and disease.

• Superiority to HaCaT: Research suggests that N/TERT-1 cells form more biologically relevant models than the widely used HaCaT cell line, particularly in terms of differentiation and response to inflammatory stimuli.

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: [email protected]

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