BioVector? Lact-C2-GFP質(zhì)粒是一種表達(dá)載體，用作可視化活細(xì)胞中磷脂酰絲氨酸（PS）的生物傳感器。它表達(dá)一個(gè)融合蛋白，該融合蛋白由牛源性乳粘連蛋白（Lactadherin）的C2結(jié)構(gòu)域（Lact-C2）連接到綠色熒光蛋白（GFP）組成。

• 磷脂酰絲氨酸（PS）檢測(cè)： 該質(zhì)粒的主要功能是定位并追蹤PS。PS是一種重要的陰離子磷脂，在健康細(xì)胞中通常位于質(zhì)膜的內(nèi)（胞質(zhì)）小葉。它暴露在外小葉通常是細(xì)胞凋亡（程序性細(xì)胞死亡）和其他生物過程的信號(hào)。

• GFP融合蛋白： 表達(dá)的GFP-Lact-C2融合蛋白以鈣非依賴性方式高親和力、特異性地結(jié)合PS。當(dāng)在細(xì)胞中表達(dá)時(shí)，GFP標(biāo)簽允許研究人員使用熒光顯微鏡觀察細(xì)胞內(nèi)PS的位置。

• 定位： 該探針主要標(biāo)記質(zhì)膜，但也可以裝飾內(nèi)體等內(nèi)部細(xì)胞器，反映了PS在這些不同細(xì)胞隔室中的存在。

• 特異性： C2結(jié)構(gòu)域特異性地與PS相互作用，并對(duì)不同膜環(huán)境中的PS具有選擇性，使其成為區(qū)分PS與其他磷脂的可靠工具。

主要特點(diǎn)

• 插入基因： 該質(zhì)粒包含牛源性乳粘連蛋白（MFGE8基因）的C2結(jié)構(gòu)域和綠色熒光蛋白（GFP）的編碼序列。

• 應(yīng)用： 它廣泛應(yīng)用于細(xì)胞生物學(xué)研究，特別是活細(xì)胞成像，用于研究膜動(dòng)力學(xué)、細(xì)胞凋亡和蛋白質(zhì)定位。

• 表達(dá)： 它可用于哺乳動(dòng)物細(xì)胞中的瞬時(shí)表達(dá)，或在DH5alpha等大腸桿菌菌株中擴(kuò)增。

• 標(biāo)記物： 根據(jù)具體的載體骨架（例如，p416或pEGFP-C2），它通常賦予細(xì)菌抗生素抗性（如氨芐青霉素或卡那霉素）以供篩選，并可能具有用于真核細(xì)胞穩(wěn)定表達(dá)的選擇標(biāo)記物（如G418抗性）。

• Phosphatidylserine (PS) Detection: The primary function of the plasmid is to localize and track PS, an important anionic phospholipid that is typically found on the inner (cytosolic) leaflet of the plasma membrane in healthy cells. Its exposure on the outer leaflet often serves as a signal for apoptosis (programmed cell death) and other biological processes.

• GFP Fusion Protein: The expressed GFP-Lact-C2 fusion protein binds with high affinity and specificity to PS in a calcium-independent manner. When expressed in cells, the GFP tag allows researchers to visualize the location of PS within the cell using fluorescence microscopy.

• Localization: The probe primarily labels the plasma membrane, but it can also decorate internal organelles like endosomes, reflecting the presence of PS in these various cellular compartments.

• Specificity: The C2 domain specifically interacts with PS and is selective for PS in different membrane environments, making it a reliable tool for distinguishing PS from other phospholipids.

Key Features

• Insert Gene: The plasmid contains the coding sequences for the C2 domain of bovine lactadherin (MFGE8 gene) and the Green Fluorescent Protein (GFP).

• Applications: It is widely used in cell biology research, particularly for live-cell imaging, to study membrane dynamics, apoptosis, and protein localization.

• Expression: It can be used for transient expression in mammalian cells or propagated in E. coli strains like DH5alpha.

• Markers: Depending on the specific vector backbone (e.g., p416 or pEGFP-C2), it typically confers antibiotic resistance (like ampicillin or kanamycin) for selection in bacteria and may have selection markers (like G418 resistance) for stable expression in eukaryotic cells.

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

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