NTCC?

• 起源： 該細胞系源自人胚胎腎293（HEK293）細胞。

• 功能： 它作為逆轉(zhuǎn)錄病毒包裝細胞系發(fā)揮作用，設(shè)計用于在誘導后產(chǎn)生慢病毒載體顆粒。

• 誘導系統(tǒng)： 該系統(tǒng)使用Tet-off誘導啟動子系統(tǒng)。在抗生素強力霉素（Dox）存在時，基因表達被抑制。撤除Dox后，病毒成分（包裝蛋白和VSV-G包膜蛋白）的產(chǎn)生被激活。

• 安全性： 它被開發(fā)用于生成高效價的自失活（SIN）載體，與早期的慢病毒系統(tǒng)相比，這些載體具有更高的安全性，因為它們在靶細胞中保留了自失活特性。

• 增強效果： 添加丁酸鈉（一種脫乙?；种苿┛赏ㄟ^影響染色質(zhì)重塑，顯著提高基因表達水平和產(chǎn)生的病毒滴度。

• 應(yīng)用： SODk1細胞產(chǎn)生的載體可以有效轉(zhuǎn)導分裂細胞和非分裂細胞，包括大鼠大腦中終末分化的神經(jīng)元，這使得它們在體內(nèi)基因遞送研究中非常有價值

• Origin: The cell line is derived from human embryonic kidney 293 (HEK293) cells.

• Function: It functions as a retrovirus packaging cell line, designed to produce lentiviral vector particles upon induction.

• Inducible System: The system uses a Tet-off inducible promoter system. In the presence of the antibiotic doxycycline (Dox), gene expression is suppressed. Upon Dox withdrawal, the production of viral components (packaging proteins and the VSV-G envelope protein) is activated.

• Safety: It was developed to generate high-titer SIN (self-inactivating) vectors, which have enhanced safety features compared to earlier lentiviral systems as they retain their self-inactivating properties in target cells.

• Enhancement: The addition of sodium butyrate, a deacetylation inhibitor, significantly increases the magnitude of gene expression and the resulting vector titers by affecting chromatin remodeling.

• Application: The vectors produced by SODk1 cells can efficiently transduce both dividing and non-dividing cells, including terminally differentiated neurons in rat brains, making them valuable for in vivo gene delivery studies.

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: [email protected]

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