The NTCC? BT-C cell line is one of several established bovine trophoblast cell lines (BT-A to BT-L) used as an in vitro model for studying the development and function of the bovine placenta and the maternal-fetal interface.

Key Characteristics and Use

• Origin: The cell lines (BT-A to BT-L) were established from in vitro fertilized bovine embryos.

• Purpose: BT-C and related cell lines are valuable tools for researchers because they can differentiate into mature trophoblast cells, including the formation of characteristic binucleate giant cells, under specific culture conditions.

• Differentiation: Trophoblast cells play a pivotal role in the establishment of pregnancy and placental development in cattle. These cell lines express key markers such as cytokeratin, and can be induced to express pregnancy-associated proteins like placental lactogen (PL1), prolactin-related protein-1 (PRP1), and pregnancy-associated glycoprotein-1 (PAG1).

• Research Applications: They are used in studies involving trophoblast differentiation, binucleation (the process of forming binucleate cells which then fuse with uterine epithelial cells), and the regulation of cell invasiveness.

• Specific Properties: In one study examining the expression of the gene SOLD1, the BT-C cell line was noted to have one of the lowest expression levels of the gene among the lines examined, and its invasiveness was not affected by anti-bovine SOLD1 antibodies, unlike the BT-K line. This highlights the variable characteristics among different BT cell lines.

• Handling: BT-C cells are considered somewhat difficult to handle compared to typical cancer or fibroblast cell lines.

• Growth: They grow in a colony-like fashion and can form dome-like structures (vesicles).

• Maintenance: Special attention to culture techniques is necessary, such as not fully dissociating the cells during passaging and using an increased concentration of Fetal Bovine Serum (FBS) during the freeze-thaw process

• 起源： BT-C細(xì)胞系（JCRB1866）建立自體外受精的牛胚泡（胚囊）滋養(yǎng)層。

• 用途： 它是研究牛滋養(yǎng)層細(xì)胞分化、胎盤發(fā)育以及早期妊娠失敗機(jī)制的重要工具。研究人員利用這些細(xì)胞系探討滋養(yǎng)層細(xì)胞的侵襲性（invading ability）調(diào)控等過程。

• 分化能力： 在特定的培養(yǎng)條件下，BT-C細(xì)胞可以分化成成熟的滋養(yǎng)層細(xì)胞，包括形成特征性的雙核巨細(xì)胞（binucleate giant cells）。

• 標(biāo)記物表達(dá)： 這些細(xì)胞表達(dá)細(xì)胞角蛋白（cytokeratin）等關(guān)鍵滋養(yǎng)層標(biāo)記物，并且可以被誘導(dǎo)表達(dá)妊娠相關(guān)蛋白，例如胎盤生乳素（PL1）、催乳素相關(guān)蛋白-1（PRP1）和妊娠相關(guān)糖蛋白-1（PAG1）。

• 科研價(jià)值： BT-C細(xì)胞系有助于科學(xué)家了解牛胚胎著床前后的復(fù)雜信號(hào)傳導(dǎo)和母胎對(duì)話過程。

• 培養(yǎng)難度： 相比于常見的癌細(xì)胞系或成纖維細(xì)胞系，BT-C細(xì)胞系通常被認(rèn)為處理起來“有些難度”。

• 生長方式： 它們傾向于以克?。洌┓绞缴L，并能形成圓頂狀結(jié)構(gòu)（囊泡）。

• 維護(hù)要求： 在傳代過程中，需要注意培養(yǎng)技術(shù)，例如不應(yīng)將細(xì)胞完全解離成單個(gè)細(xì)胞，并在凍存/復(fù)蘇過程中使用更高濃度的胎牛血清（FBS）。

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

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