KGN NTCC?永生化人卵巢顆粒樣瘤細胞株immortalized human ovarian granulosa-like tumor cell line

價　　格：￥99860
貨　　號：NTCC?-KGN CELL
產(chǎn)　　地：北京

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NTCC? KGN cell line is an immortalized human ovarian granulosa-like tumor cell line widely used as an in vitro model for studying ovarian function, hormone regulation, and diseases like polycystic ovary syndrome (PCOS) and ovarian cancer.

KGN Cells

Key Characteristics

Origin: Derived from a stage III ovarian granulosa cell tumor from a 63-year-old Japanese woman.
Morphology and Growth: Adherent cells with a fibroblast-like morphology and a relatively slow population doubling time of around 46.4 hours.
Functionality: KGN cells retain key physiological characteristics of normal granulosa cells, including the expression of functional follicle-stimulating hormone (FSH) receptors and steroidogenic activity. They can produce progesterone and estrogen, particularly after stimulation with agents like forskolin or dibutyryl cyclic adenosine monophosphate (dbcAMP).
Genetic Profile: The cell line has an abnormal karyotype (45, XX, 7q-, -22) and features a common somatic mutation in the FOXL2 gene (p.Cys134Trp, or c.402C>G), which is found in almost all adult-type granulosa cell tumors.
Biosafety Level: Biosafety Level 1.

Research Applications

Due to their unique properties, KGN cells serve as a valuable research tool for:

Investigating the molecular mechanisms of ovarian follicle development and function.
Studying hormonal signaling pathways, especially those related to FSH and steroidogenesis.
Modeling reproductive and endocrine disorders, such as PCOS.
Exploring potential therapeutic targets for ovarian granulosa cell tumors and testing anti-cancer drugs.

Researchers should be aware that KGN cells have some unique features compared to primary granulosa cells, such as extremely high expression of the antioxidant gene TXNRD1, which might affect studies focused on the interplay between steroidogenesis, reactive oxygen species, and antioxidants.

NTCC? KGN細胞系是一種永生化人卵巢顆粒樣瘤細胞系，被廣泛用作研究卵巢功能、激素調節(jié)以及多囊卵巢綜合征（PCOS）和卵巢癌等疾病的體外模型。

主要特點

起源：源自一名63歲日本女性的III期卵巢顆粒細胞瘤。
形態(tài)和生長：貼壁細胞，具有成纖維細胞樣形態(tài)，倍增時間相對較慢，約為46.4小時。
功能性： KGN細胞保留了正常顆粒細胞的關鍵生理特征，包括表達功能性促卵泡激素（FSH）受體和類固醇生成活性。它們可以產(chǎn)生孕酮和雌激素，特別是在用福司可林（forskolin）或二丁酰環(huán)磷酸腺苷（dbcAMP）等試劑刺激后。
基因概況：該細胞系具有異常核型（45, XX, 7q-, -22），并具有FOXL2基因中常見的體細胞突變（p.Cys134Trp，或c.402C>G），該突變幾乎存在于所有成人型顆粒細胞瘤中。
生物安全級別：生物安全一級。