• Source: The parental NuTu-19 line was developed from a spontaneous ovarian surface epithelial cell tumor in a female Fischer 344 rat.

• Cell Type: Rat ovarian adenocarcinoma epithelial cell line.

• Luciferase Expression: The NuTu-19-luc line has been transduced to constitutively express the firefly luciferase gene. This genetic modification allows the cells to produce a bioluminescent signal, which can be detected with specialized imaging equipment when the substrate luciferin is added.

• In vivo cancer research: The cells can be injected into laboratory animals (e.g., rats) to create a tumor model. The resulting tumors can then be monitored non-invasively and in real-time using bioluminescence imaging (BLI).

• Metastasis tracking: The bioluminescent signal allows researchers to quantitatively track tumor growth, progression, and metastasis within a living animal.

• Drug screening and therapeutic efficacy: The response of tumors to various treatments can be quantitatively assessed over time by measuring changes in the luciferase signal. This provides a high-throughput method for evaluating the efficacy of new cancer therapies.

• Oncology studies: More broadly, the cell line is a tool for studying tumorigenesis, apoptosis, and other biological processes in the context of ovarian cancer.

• 來(lái)源: 親代 NuTu-19 細(xì)胞系來(lái)源于雌性 Fischer 344 大鼠的自發(fā)性卵巢表面上皮細(xì)胞腫瘤。

• 細(xì)胞類(lèi)型: 大鼠卵巢腺癌上皮細(xì)胞系。

• 熒光素酶表達(dá): NuTu-19-luc 細(xì)胞系經(jīng)過(guò)轉(zhuǎn)染，可以組成型地表達(dá)螢火蟲(chóng)熒光素酶基因。這種基因改造使細(xì)胞能夠產(chǎn)生生物發(fā)光信號(hào)，當(dāng)加入底物熒光素后，可以通過(guò)專(zhuān)門(mén)的成像設(shè)備進(jìn)行檢測(cè)。

• 體內(nèi)癌癥研究: 研究人員可以將這種細(xì)胞注入實(shí)驗(yàn)動(dòng)物（如大鼠）體內(nèi)，建立腫瘤模型。隨后，利用生物發(fā)光成像（BLI）技術(shù)，可以對(duì)形成的腫瘤進(jìn)行實(shí)時(shí)、無(wú)創(chuàng)的監(jiān)測(cè)。

• 轉(zhuǎn)移追蹤: 生物發(fā)光信號(hào)使研究人員能夠在活體動(dòng)物體內(nèi)定量追蹤腫瘤的生長(zhǎng)、進(jìn)展和轉(zhuǎn)移情況。

• 藥物篩選和療效評(píng)估: 通過(guò)測(cè)量熒光素酶信號(hào)隨時(shí)間的變化，可以定量評(píng)估腫瘤對(duì)各種治療的反應(yīng)。這為評(píng)估新癌癥療法的有效性提供了一種高通量的方法。

• 腫瘤學(xué)研究: 更廣泛地說(shuō)，該細(xì)胞系是研究卵巢癌發(fā)生、凋亡和其他生物過(guò)程的有用工具

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: [email protected]