The NTCC? cho-k1/aequorin/ga16 cell line is a genetically engineered Chinese Hamster Ovary (CHO-K1) cell line used as a biosensor to measure changes in intracellular calcium levels. It is used in research and drug discovery to study G-protein coupled receptors (GPCRs) and other signaling pathways.

Components and function

• CHO-K1 host cells: A subclone of the original CHO cell line, CHO-K1 cells are a standard host for producing recombinant proteins and are easily transfected with foreign DNA. They are of Chinese hamster ovarian origin and require proline in their growth medium.

• Aequorin: A photoprotein that emits light in the presence of calcium. In the cho-k1/aequorin/ga16 line, the aequorin gene is stably expressed and targeted to a specific cellular location, such as the mitochondria, to measure localized calcium changes.

• Ga16: A chimeric G-protein alpha subunit that couples a wide range of receptors to the phospholipase C pathway, resulting in increased intracellular calcium. By co-expressing Ga16, the cell line can amplify the calcium response from various GPCRs.

• GPCR screening: Because the Ga16 protein couples with many GPCRs, the cell line can be used to screen for compounds that activate these receptors, making it a versatile tool in early-stage drug discovery.

• Calcium signaling studies: The aequorin reporter system allows for the detection of subtle or localized changes in intracellular calcium, which can provide insight into complex cellular signaling pathways.

• Mitochondrial calcium dynamics: In versions where aequorin is targeted to the mitochondria, the cell line can be used to specifically investigate mitochondrial calcium influx and its role in cell death and metabolism.

• CHO-K1宿主細(xì)胞： 標(biāo)準(zhǔn)的重組蛋白生產(chǎn)宿主細(xì)胞系，易于轉(zhuǎn)染外源DNA。它源自中國(guó)倉(cāng)鼠卵巢，需要在培養(yǎng)基中添加脯氨酸才能生長(zhǎng)。

• 水母素（Aequorin）： 一種生物發(fā)光蛋白質(zhì)，在鈣離子存在時(shí)會(huì)發(fā)光。在 cho-k1/aequorin/ga16 細(xì)胞系中，水母素基因被穩(wěn)定表達(dá)，并被靶向到特定的細(xì)胞位置（如線粒體），以測(cè)量局部鈣離子的變化。

• Ga16： 一種嵌合G蛋白alpha亞基，可將各種受體與磷脂酶 C 通路偶聯(lián)，從而導(dǎo)致細(xì)胞內(nèi)鈣離子增加。通過(guò)共表達(dá) Ga16，該細(xì)胞系可以放大來(lái)自不同 GPCR 的鈣離子反應(yīng)。

• GPCR篩選： 由于 Ga16 蛋白與多種 GPCR 偶聯(lián)，該細(xì)胞系可用于篩選激活這些受體的化合物，使其成為早期藥物研發(fā)中一種多功能工具。

• 鈣信號(hào)研究： 水母素報(bào)告系統(tǒng)能夠檢測(cè)細(xì)胞內(nèi)鈣離子細(xì)微或局部的變化，從而深入了解復(fù)雜的細(xì)胞信號(hào)通路。

• 線粒體鈣動(dòng)力學(xué)： 在水母素被靶向到線粒體的版本中，該細(xì)胞系可專門用于研究線粒體鈣內(nèi)流及其在細(xì)胞死亡和代謝中的作用。

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: [email protected]

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