NTCC? EY917 酵母菌株是一種經(jīng)過(guò)基因工程改造的釀酒酵母（Saccharomyces cerevisiae）突變菌株，專(zhuān)門(mén)用于磷酸鹽轉(zhuǎn)運(yùn)研究。它是研究來(lái)自其他生物（特別是植物和真菌）的磷酸鹽轉(zhuǎn)運(yùn)蛋白功能的關(guān)鍵工具。

主要特點(diǎn)

EY917 菌株的決定性特征是其磷酸鹽轉(zhuǎn)運(yùn)系統(tǒng)有缺陷。這是通過(guò)敲除酵母基因組中所有已知的磷酸鹽高親和力和低親和力轉(zhuǎn)運(yùn)蛋白基因來(lái)實(shí)現(xiàn)的。具體來(lái)說(shuō)，該菌株缺失了五個(gè)關(guān)鍵的磷酸鹽轉(zhuǎn)運(yùn)蛋白基因：

• pho84Δ

• pho87Δ

• pho89Δ

• pho90Δ

• pho91Δ

由于缺乏這些功能性轉(zhuǎn)運(yùn)蛋白，EY917 菌株無(wú)法有效地從環(huán)境中吸收無(wú)機(jī)磷酸鹽（Pi）。這導(dǎo)致當(dāng)其在低磷酸鹽濃度的培養(yǎng)基中培養(yǎng)時(shí)，生長(zhǎng)非常差。

研究應(yīng)用

EY917 菌株的主要用途是進(jìn)行互補(bǔ)性試驗(yàn)，以測(cè)試來(lái)自其他生物的基因是否能作為磷酸鹽轉(zhuǎn)運(yùn)蛋白發(fā)揮作用。實(shí)驗(yàn)過(guò)程如下：

1. 將來(lái)自植物、真菌或其他生物的基因（候選基因）克隆到質(zhì)粒中。

2. 然后將該質(zhì)粒轉(zhuǎn)化到 EY917 突變菌株中。

3. 在低磷酸鹽培養(yǎng)基中培養(yǎng)轉(zhuǎn)化的 EY917 細(xì)胞。

如果候選基因成功編碼了一個(gè)功能性的磷酸鹽轉(zhuǎn)運(yùn)蛋白，它將恢復(fù)酵母吸收磷酸鹽的能力，使得 EY917 菌株能夠正常生長(zhǎng)或比未轉(zhuǎn)化的菌株生長(zhǎng)得更好。如果該基因不具有轉(zhuǎn)運(yùn)功能，酵母的生長(zhǎng)將依然很差。這提供了一種直接且簡(jiǎn)單的方法，用于篩選和表征來(lái)自各種來(lái)源的磷酸鹽轉(zhuǎn)運(yùn)蛋白基因的功能。

The EY917 yeast strain is a genetically engineered mutant strain of Saccharomyces cerevisiae (baker's yeast) that is specifically used in research on phosphate transport. It is a crucial tool for studying the function of phosphate transporters from other organisms, particularly plants and fungi.

Key Characteristics

The defining feature of the EY917 strain is that it has a defective phosphate transport system. This is achieved by deleting the genes for all known high-affinity and low-affinity phosphate transporters in the yeast genome. Specifically, the strain is a knockout for five key phosphate transporter genes:

• pho84Δ

  
  

• pho87Δ

  
  

• pho89Δ

  
  

• pho90Δ

• pho91Δ

Because it lacks these functional transporters, the EY917 strain is unable to effectively take up inorganic phosphate (Pi) from its environment. This results in very poor growth when cultured in media with low phosphate concentrations.

Research Applications

The primary use of the EY917 strain is in complementation assays to test whether a gene from another organism can function as a phosphate transporter. The experimental process works as follows:

1. A gene from a plant, fungus, or other organism (the candidate gene) is cloned into a plasmid.

2. This plasmid is then transformed into the EY917 mutant strain.

3. The transformed EY917 cells are grown in a low-phosphate medium.

If the candidate gene successfully encodes a functional phosphate transporter, it will restore the yeast's ability to absorb phosphate, allowing the EY917 strain to grow normally or grow significantly better than the untransformed strain. If the gene is not a functional transporter, the yeast will continue to show poor growth. This provides a direct and simple way to screen for and characterize the function of phosphate transporter genes from a wide variety of sources.

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