NTCC? 804G.003A 大鼠尿路上皮細(xì)胞系

804G.003A 細(xì)胞系是一種轉(zhuǎn)化過的大鼠尿路上皮細(xì)胞系，來源于患有膀胱鱗狀細(xì)胞癌的雄性 ACI 大鼠。它是其親本細(xì)胞系 804 G 的一個亞克隆，是細(xì)胞生物學(xué)研究中廣泛使用的模型，特別是在研究上皮細(xì)胞粘附和特殊連接結(jié)構(gòu)形成方面。

主要特點(diǎn)

• 來源: 該細(xì)胞系是利用致癌物 N-丁基-N-(3-羧丙基)亞硝胺 (BCPN) 誘導(dǎo)產(chǎn)生的大鼠膀胱腫瘤中建立的。

• 細(xì)胞類型: 它是一種尿路上皮細(xì)胞系，起源于尿路內(nèi)壁的上皮組織。

• 粘附特性: 804G.003A 細(xì)胞系的一個決定性特征是其形成半橋粒的能力。半橋粒是復(fù)雜的細(xì)胞結(jié)構(gòu)，用于將上皮細(xì)胞錨定在其下方的基底膜上。這使其成為研究這些細(xì)胞粘附復(fù)合物的組裝、調(diào)節(jié)和功能的經(jīng)典模型。

• 永生化: 這些細(xì)胞是經(jīng)過轉(zhuǎn)化的，這意味著它們的壽命得以延長，可以無限期地進(jìn)行培養(yǎng)，這與原代細(xì)胞不同。

研究應(yīng)用

804G.003A 細(xì)胞系是細(xì)胞和分子生物學(xué)研究人員的重要工具。其主要應(yīng)用包括：

• 研究細(xì)胞粘附: 它是研究半橋粒形成分子機(jī)制以及其中涉及的整合素和斑珠蛋白等蛋白質(zhì)的經(jīng)典模型。

• 上皮生物學(xué): 研究人員用它來了解尿路上皮的結(jié)構(gòu)和功能，尿路上皮是構(gòu)成尿路內(nèi)壁的特殊上皮組織。

• 癌癥研究: 作為一種腫瘤來源的細(xì)胞系，它可用于研究膀胱癌發(fā)展過程中細(xì)胞粘附和生長所發(fā)生的變化。

The 804G.003A cell line is a transformed rat urothelial cell line derived from a male ACI rat with a urinary bladder squamous cell carcinoma. It is a subclone of the parent 804 G cell line and is a widely used model in cell biology research, especially for studying epithelial cell adhesion and the formation of specialized junctions.

Key Characteristics

• Origin: The cell line was established from a rat urinary bladder tumor induced by a carcinogen, specifically N-butyl-N-(3-carboxypropyl)nitrosamine (BCPN).

• Cell Type: It is a urothelial cell line, which means it originates from the lining of the urinary bladder.

• Adhesion Properties: A defining characteristic of the 804G.003A cell line is its ability to form hemidesmosomes, which are complex structures that anchor epithelial cells to the underlying basement membrane. This makes it a primary model for studying the assembly, regulation, and function of these cell adhesion complexes.

• Immortalization: The cells are transformed, meaning they have a prolonged lifespan and can be cultured indefinitely, unlike primary cells.

Research Applications

The 804G.003A cell line is a fundamental tool for researchers in cell and molecular biology. Its main applications include:

• Studying Cell Adhesion: It is a classic model for investigating the molecular mechanisms of hemidesmosome formation and the proteins involved, such as integrins and plectin.

• Epithelial Biology: Researchers use it to understand the structure and function of the urothelium, the specific type of epithelial tissue that lines the urinary tract.

• Cancer Research: As a tumor-derived cell line, it can be used to study the changes in cell adhesion and growth that occur during the development of bladder cancer.

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