The CEM.NKR-CCR5 cell line is a human T-lymphoblastoid cell line that is widely used in HIV/AIDS research. It's an engineered cell line, meaning it was modified from its parent line to express a specific protein.

Key Characteristics:

• Parent Cell Line: The original line is CEM.NKR, which is a clone of the well-known CEM cell line. A key feature of the parent CEM.NKR cells is their resistance to natural killer (NK) cell-mediated lysis.

• Genetic Modification: The CEM.NKR-CCR5 cells were created by transducing the CEM.NKR parent cells with a retroviral vector to make them express human CCR5.

• CCR5 Expression: This is the most crucial characteristic of this cell line. CCR5 (C-C chemokine receptor type 5) is a coreceptor that many strains of HIV-1 use to enter and infect T cells. The parental CEM.NKR cells do not express CCR5, making them resistant to R5-tropic HIV-1 strains. By introducing CCR5, the CEM.NKR-CCR5 cells become susceptible to infection by these strains.

• HIV-1 Susceptibility: The cell line expresses both the CD4 receptor and the CCR5 coreceptor (and also the CXCR4 coreceptor from its parent line), making it susceptible to infection by both R5-tropic and X4-tropic strains of HIV-1.

Research Applications:

The NTCC? CEM.NKR-CCR5 cell line is an invaluable tool for virologists and immunologists, particularly in the study of HIV. Its primary applications include:

• HIV Neutralization Assays: This cell line is used to measure the ability of antibodies and other compounds (e.g., from patient serum) to neutralize or block HIV-1 infection. It's a key part of assays that assess how effectively a patient's immune system or a new drug can prevent the virus from entering cells.

• Antiretroviral Drug Screening: Researchers use CEM.NKR-CCR5 cells to screen for and evaluate the effectiveness of new anti-HIV drugs, especially those that target the CCR5 receptor to block viral entry.

• Studying HIV Pathogenesis: The cell line provides a consistent and reproducible model to study the early stages of HIV infection, including how the virus binds to receptors, enters the cell, and begins replication.

• Vaccine Research: It can be used to test the efficacy of vaccine candidates by measuring their ability to elicit a neutralizing antibody response that prevents HIV from infecting these cells.

NTCC? CEM.NKR-CCR5 細(xì)胞系是一種廣泛應(yīng)用于艾滋?。℉IV/AIDS）研究的人類 T 淋巴母細(xì)胞系。它是一種經(jīng)過基因工程改造的細(xì)胞系，這意味著它是在其親本細(xì)胞系的基礎(chǔ)上，通過改造來表達(dá)某種特定蛋白質(zhì)。

主要特點(diǎn)

• 親本細(xì)胞系: 原始細(xì)胞系是 CEM.NKR，它是著名的 CEM 細(xì)胞系的一個克隆株。親本 CEM.NKR 細(xì)胞的一個關(guān)鍵特征是它對自然殺傷（NK）細(xì)胞介導(dǎo)的裂解具有抵抗力。

• 基因改造: CEM.NKR-CCR5 細(xì)胞是通過逆轉(zhuǎn)錄病毒載體轉(zhuǎn)導(dǎo) CEM.NKR 親本細(xì)胞而創(chuàng)建的，使其能夠表達(dá)人類 CCR5。

• CCR5 表達(dá): 這是該細(xì)胞系最重要的特性。CCR5 (C-C 趨化因子受體5型) 是許多 HIV-1 毒株用來進(jìn)入并感染 T 細(xì)胞的共受體。親本 CEM.NKR 細(xì)胞不表達(dá) CCR5，因此對 R5 嗜性 HIV-1 毒株有抵抗力。通過引入 CCR5，CEM.NKR-CCR5 細(xì)胞變得容易被這些毒株感染。

• 對 HIV-1 的敏感性: 該細(xì)胞系同時表達(dá) CD4 受體和 CCR5 共受體（以及來自其親本細(xì)胞系的 CXCR4 共受體），因此它既能被 R5 嗜性 HIV-1 毒株感染，也能被 X4 嗜性 HIV-1 毒株感染。

研究應(yīng)用

CEM.NKR-CCR5 細(xì)胞系是病毒學(xué)家和免疫學(xué)家寶貴的工具，尤其是在 HIV 研究中。其主要應(yīng)用包括：

• HIV 中和試驗(yàn): 該細(xì)胞系用于測量抗體和其他化合物（例如來自患者血清的物質(zhì)）中和或阻斷 HIV-1 感染的能力。它是評估患者免疫系統(tǒng)或新藥物能否有效阻止病毒進(jìn)入細(xì)胞的關(guān)鍵部分。

• 抗逆轉(zhuǎn)錄病毒藥物篩選: 研究人員使用 CEM.NKR-CCR5 細(xì)胞來篩選和評估新型抗 HIV 藥物的有效性，尤其是那些靶向 CCR5 受體以阻斷病毒進(jìn)入的藥物。

• 研究 HIV 發(fā)病機(jī)制: 該細(xì)胞系提供了一個穩(wěn)定且可重復(fù)的模型，用于研究 HIV 感染的早期階段，包括病毒如何與受體結(jié)合、進(jìn)入細(xì)胞并開始復(fù)制。

• 疫苗研究: 它可以用于測試候選疫苗的效力，通過測量它們引發(fā)中和抗體反應(yīng)的能力，以防止 HIV 感染這些細(xì)胞。

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

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