BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
- 價(jià) 格：￥99850
- 貨 號(hào)：BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作微信:1843439339 (QQ同號(hào))
手機(jī)：18901268599
地址：北京
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BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
| Comment | A hamster kidney-derived BHK cell clone stably expressing T7 RNA polymerase. | |||
| Year of deposit | 2018 | ||||
| Animal | _hamster < Mammals | ||||
| Strain name | Syrian | ||||
| Genus | Mesocricetus | ||||
| Species | auratus | ||||
| Gender | Unknown | ||||
| Tissue | kidney | ||||
| Classification | other | ||||
| Recombinant | recombinant | ||||
| Exogene | T7 RNA polymerase gene | ||||
| 2.9K base | |||||
| Vector | pCAGGS/MCS | ||||
| Selection | 600 μg/ml hygromycin (every 10 passages) | ||||
| Lifespan | infinite | ||||
| Morphology | epithelial-like | ||||
| Cellosaurus(Expasy) | CVCL_A8V7 | ||||
| Medium | Medium List | ||||
| Culture type | Adherent cells | Adherent cells | |||
| Culture medium | Eagle’s MEM with 5% Fetal Calf Serum and 10% Tryptose Phosphate Broth (BD, Difco 260300) | MEM + 5% FBS + 10% Tryptose Phosphate Broth ( Media preparation ) | |||
| Antibiotics | 100 U/ml penicillin and 100 U/ml streptomycin | Free | |||
| Passage method | Trypsin-EDTA | 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA) | |||
| Designated culture dish | 100 mm dish | ||||
| Culture information | Passage ratio | 1:5 – 1:10 | 1 : 8 split | ||
| SC frequency | Subculture : once/4 days | Subculture : once/4 days | |||
| Temperature | 37 ℃ | 37 ℃ | |||
| CO2 concentration | 5 % | 5 % | |||
| Freeze medium | CELLBANKER 1 | CELLBANKER 1 | |||
| Freezing method | Slow freezing | ||||
| Mycoplasma/Acholeplasma | (-) | ||||
| Mycoplasma | (-) | ||||
| Animal PCR | OK | ||||
| Images | |||||
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生產(chǎn)廠家Supplier:
BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
E-mail:[email protected]
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