pJAZZ-OK BioVector?高難度和不穩(wěn)定DNA克隆載體

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

BioVector? pJAZZ-OK is a specialized linear bacterial vector designed for cloning and maintaining DNA sequences that are unstable or difficult to clone in traditional circular plasmids.

pJAZZOK plasmid map

Key Features:

• Linear Structure: Unlike circular plasmids, pJAZZ-OK exists as a linear molecule. This linear configuration can help stabilize challenging DNA sequences, such as those with repetitive elements or high GC content, which can lead to plasmid instability in circular forms.

• Kanamycin Resistance Gene: Contains the kanR gene, conferring resistance to the antibiotic kanamycin. This allows for the selection of bacteria that have successfully taken up the plasmid.

• Transcriptional Terminators: Features transcriptional terminators flanking the cloning site. These terminators help prevent transcription from vector sequences into the insert DNA, which can interfere with gene expression or stability.

• GC-Rich Cloning Site: The cloning site is optimized for GC-rich DNA sequences, making it suitable for cloning challenging DNA fragments.

Applications:

• Cloning of Unstable DNA Sequences: Ideal for cloning repetitive DNA, GC-rich sequences, or other DNA fragments that are prone to rearrangements or deletions in circular plasmids.

• Genomic Libraries: Used to construct genomic libraries, especially those enriched for GC-rich or repetitive DNA.

• Metagenomics: Employed in metagenomic studies to clone and analyze diverse microbial DNA.

Significance:

pJAZZ-OK and other linear cloning vectors have expanded the capabilities of molecular biology techniques. By providing a stable platform for cloning challenging DNA sequences, these vectors have enabled researchers to study and manipulate a wider range of genetic material.

Map

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BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

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