BioVector? pAM401糞腸球菌穿梭表達(dá)質(zhì)粒載體

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

pAM401 is a plasmid commonly used in genetic engineering. Here's a breakdown:

• Plasmid: A small, extrachromosomal DNA molecule that can replicate independently of the host chromosome.

• Shuttle Vector: pAM401 is a type of shuttle vector, meaning it can replicate in two different types of organisms: Escherichia coli (E. coli) and Enterococcus faecalis. This versatility makes it useful for transferring genes between these bacteria.

• Key Features:

  • Origin of Replication: Contains origins of replication for both E. coli and E. faecalis, allowing it to replicate in both hosts.

  • Antibiotic Resistance Genes: Carries genes that confer resistance to antibiotics, such as chloramphenicol and tetracycline. These genes are used for selecting bacteria that have successfully taken up the plasmid.

  • Multiple Cloning Sites (MCS): Contains a region with various restriction enzyme recognition sites, allowing researchers to easily insert foreign DNA fragments into the plasmid.

  
  

Applications:

• Gene Cloning: Used to clone and amplify genes of interest.

• Gene Expression: Can be used to express cloned genes in E. faecalis.

• Genetic Analysis: Used to study gene function and regulation in E. faecalis.

Significance:

pAM401 and its derivatives have been valuable tools in the study of Enterococcus faecalis, a bacterium that can cause serious infections in humans. By allowing researchers to manipulate the genes of E. faecalis, these plasmids have contributed to our understanding of this important pathogen and the development of new strategies to combat infections.

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