• Vector backbone
  pHAGE-DEST

• Backbone size w/o insert (bp)6000

• Total vector size (bp)12336

• Vector type
  Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  St1 dCas9
• Species
  Synthetic
• Insert Size (bp)
  3357

• PromoterCMV-TO

• Tag / Fusion Protein

  • 3XsfGFP

  
  

Cloning Information

• Cloning methodRestriction Enzyme

• 5′ cloning siteEcoRI (not destroyed)

• 3′ cloning siteXbaI (not destroyed)

• 5′ sequencing primercgaaggaatagaagaagaaggtgga

• 3′ sequencing primerCCAAAGGGAGATCCGACTCG