• Vector backbone
  pBJ1

• Backbone size w/o insert (bp)6326

• Total vector size (bp)8590

• Vector type
  Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Growth instructions
  No problems with this plasmid. Can use standard strains for growth.
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  ClopHensorN
• Alt name
  E2GFP fused to Tandem Tomato
• Species
  Aq. Victoria and Discosoma sp
• Insert Size (bp)
  2264
• Mutation
  E2GFP is EGFP-T203Y

• PromoterCAG

• Tag / Fusion Protein

  • Strep-II (N terminal on insert)

  
  

Cloning Information

• Cloning methodRestriction Enzyme

• 5′ cloning siteNot1 (not destroyed)

• 3′ cloning siteHindIII (not destroyed)

• 5′ sequencing primerpCAG_F_primer