• Vector backbone
  pENTR4
• Backbone manufacturer
  Invitrogen

• Backbone size w/o insert (bp)2477

• Vector type
  Entry vector

Growth in Bacteria

• Bacterial Resistance(s)
  Kanamycin, 50 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Growth instructions
  TOP10F', 37oC
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Mediator of DNA damage checkpoint 1
• Alt name
  MDC1
• Alt name
  NFBD1
• Alt name
  KIAA0170
• Species
  H. sapiens (human)
• Insert Size (bp)
  6269
• Entrez Gene
  MDC1(a.k.a. NFBD1)

Cloning Information

• Cloning methodRestriction Enzyme

• 5′ cloning siteSal I (not destroyed)

• 3′ cloning siteXba I (not destroyed)

• 5′ sequencing primerENTRforw

• 3′ sequencing primerENTRrev