EB66 NTCC?疫苗和治療性蛋白生產(chǎn)用鴨胚胎干細胞株duck embryonic stem cell-BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

The NTCC? EB66 cell line is a duck embryonic stem cell-derived cell line that has been developed as a platform for the production of vaccines and therapeutic proteins. It is considered a valuable tool in the biotechnology industry due to its unique characteristics and advantages.

NTCC? EB66 cell line

Key characteristics of the NTCC? EB66 cell line:

• Origin: Derived from duck embryonic stem cells

• Advantages:

  • High growth rate: Short population doubling time, allowing for rapid cell expansion.

  • High cell density: Can reach very high cell densities in culture, increasing production yields.

  • Unique metabolic profile: Low ammonium and lactate accumulation, reducing the need for frequent media changes.

  • Reduced fucose content: Leads to enhanced antibody-dependent cellular cytotoxicity (ADCC) activity of therapeutic antibodies produced in this cell line.

  • Genetic stability: Maintained through the expression of telomerase, ensuring long-term culture stability.

  • Scalability: Can be easily scaled up for industrial production in bioreactors.

  
  

Applications of the EB66 cell line:

• Vaccine production: Used for the production of a variety of human and veterinary vaccines, including influenza, rabies, and Zika virus vaccines.

• Therapeutic protein production: Used for the production of therapeutic antibodies and other recombinant proteins.

Advantages of using the EB66 cell line:

• Consistent product quality: The use of a well-characterized cell line ensures consistent product quality and reproducibility.

• Reduced production costs: The high growth rate and scalability of the EB66 cell line can lead to reduced production costs.

• Enhanced product efficacy: The reduced fucose content of antibodies produced in EB66 cells can lead to enhanced ADCC activity, improving their therapeutic efficacy.

Overall, the NTCC? EB66 cell line is a valuable tool for the production of vaccines and therapeutic proteins. Its unique characteristics and advantages make it a promising platform for the development of new and improved biopharmaceuticals.

NTCC?EB66 Cell Line Culture Techniques

EB66 cells are expanded to required pre-infection densities in BioVector? EB66Complete Medium-I (Catalog No. BioVector?-931135M1) growth medium. EB66 cellsare normally grown at 37±1^oCand 7.5% CO2 in shaker flasks at 120 rpm.

Virusinfection and production is induced with the addition of seed virus and 1.5 -1.8 times (v/v) pre-warmed BioVector? EB66 Complete Medium-II (Catalog No.BioVector?-931135MII) medium to growth medium. Standard infection celldensities are >2 x 10⁶ viable cells/mL (prior to dilution withproduction medium) at a multiplicity of infection (MOI) of 0.01 - 0.10.

Optimalconditions for virus amplification may vary depending on viral construct andprocess conditions. Recommended post-infection conditions are 33 to 37 °C and7.5 % CO2 in shaker flasks at 120 rpm.

Cryopreservation

BioVector?EB66 Complete Medium-II is not recommended for cryopreservation of the EB66cell line. Please refer to BioVector? EB66 Complete Medium-I (Catalog No.BioVector?-931135M1) growth medium literature for recommended cryopreservation conditions.

NTCC? EB66 細胞系是一種來源于鴨胚胎干細胞的細胞系，已被開發(fā)為生產(chǎn)疫苗和治療性蛋白的平臺。由于其獨特的特性和優(yōu)勢，它被認為是生物技術(shù)行業(yè)中一種有價值的工具。

NTCC?EB66 細胞系的關(guān)鍵特征：

• 起源： 源自鴨胚胎干細胞

• 優(yōu)勢：

  • 高生長速率： 細胞倍增時間短，可快速擴增細胞。

  • 高細胞密度： 在培養(yǎng)中可達到非常高的細胞密度，從而提高產(chǎn)量。

  • 獨特的代謝特征： 氨和乳酸積累較少，減少了頻繁更換培養(yǎng)基的需求。

  • 降低巖藻糖含量： 導(dǎo)致在該細胞系中產(chǎn)生的治療性抗體的抗體依賴性細胞毒性 (ADCC) 活性增強。

  • 遺傳穩(wěn)定性： 通過端粒酶的表達維持，確保長期培養(yǎng)穩(wěn)定性。

  • 可擴展性： 可在生物反應(yīng)器中輕松放大生產(chǎn)。

  
  

EB66 細胞系的應(yīng)用：

• 疫苗生產(chǎn)： 用于生產(chǎn)多種人類和獸用疫苗，包括流感、狂犬病和寨卡病毒疫苗。

• 治療性蛋白生產(chǎn)： 用于生產(chǎn)治療性抗體和其他重組蛋白。

使用 EB66 細胞系的優(yōu)勢：

• 一致的產(chǎn)品質(zhì)量： 使用經(jīng)過充分表征的細胞系可確保一致的產(chǎn)品質(zhì)量和可重復(fù)性。

• 降低生產(chǎn)成本： EB66 細胞系的高生長速率和可擴展性可降低生產(chǎn)成本。

• 提高產(chǎn)品功效： 在 EB66 細胞中產(chǎn)生的抗體巖藻糖含量降低可提高 ADCC 活性，從而提高其治療功效。

總的來說，NTCC? EB66 細胞系是生產(chǎn)疫苗和治療性蛋白的有價值工具。其獨特的特性和優(yōu)勢使其成為開發(fā)新型和改進型生物制藥產(chǎn)品的有前途的平臺。

生產(chǎn)廠家Supplier:

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

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