BioVector? pHTN HaloTag CMV-neo Vector哺乳動物HaloTag蛋白標(biāo)簽融合表達載體

N-Terminal Vector for Traditional Cloning

The BioVector? pHTN HaloTag CMV-neo Vector is configured to append the HaloTag? tag to the amino-terminus of the fused protein. The vector provides constitutive high-level protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate-early enhancer/promoter. The vector contains a multiple cloning region for convenient cloning, and can be used for both transient and stable gene expression. The stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the antibiotic G-418 (Cat.# V7983). Alternatively, the HaloTag? coding region and the accompanying linker can be removed (either using PCR or restriction enzyme digestion) and transferred into other commercially available vectors.

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