TK2420大腸桿菌突變株NTCC? E.coli缺乏Kdp， Trk和Kup K+吸收系統(tǒng)-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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TK2420大腸桿菌突變株NTCC? E.coli缺乏Kdp， Trk和Kup K+吸收系統(tǒng)

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

TK2420 is a mutant strain of Escherichia coli that is deficient in the Kdp, Trk, and Kup K+ uptake systems:

K+ requirement
TK2420 requires exogenous K+ to maintain steady-state pH homeostasis and to recover from sudden acid shifts.
Complementation
The recombinant plasmid pGerN, which expresses the endospore germination protein GerN, can complement the phenotype of TK2420. This suggests that GerN may catalyze both Na+ extrusion and K+ uptake.
Growth inhibition
Aa-KefB and Aa-NhaP inhibit the growth of TK2420, suggesting that they catalyze K+ efflux.
K+ uptake
K+ uptake by TK2420 yggS-T/pUT is slightly decreased by the addition of 5mM NaCl.
Osmolarity
The K+ requirement of TK2420 is partly compensated by supplementation with NaCl, choline chloride, proline, or sucrose. This suggests that the K+ requirement is mediated in part by osmolarity
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