[Designation名稱] NTCC? CRISPRi Gen1C 6 Cell Line細(xì)胞株
BioVector NTCC典型培養(yǎng)物保藏中心

SY WTC CRISPRi Gen1 clone 6; 1C-6
DR Wikidata; Q93458765
RX PubMed=26971820;
CC Population: Japanese.
CC Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
CC Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC Cell type: Fibroblast of skin; CL=CL_0002620.
OX NCBI_TaxID=9606; ! Homo sapiens (Human)
HI CVCL_Y803 ! GM25256
[Organism物種來源]
[Disease疾病種類]
[Accession編號] NTCC?-AC CVCL_VM39
[Comments描述]
[Reference參考文獻(xiàn)]
[Category分類]
[Date保藏時間]
[Synonyms別名]
[Hierarchy]
培養(yǎng)方法：
Medium培養(yǎng)基：NTCC? CRISPRi Gen1C 6 Complete Medium
Growth properties形態(tài)：貼壁/懸浮：
Culture Condition培養(yǎng)條件：Temperature溫度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生產(chǎn)廠家: BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
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