BioVector? NTCC? pMK289 (mAID-mClover-NeoR)哺乳動物基因編輯質(zhì)粒BioVector?載體

pMK289 (mAID-mClover-NeoR)
(Plasmid BioVector-72827)
Purpose
C-terminal tagging with mAID-mClover using CRISPR/Cas9

Vector backbonepBluescript KS(-)
Backbone size w/o insert (bp)3242
Total vector size (bp)6117
Vector typeMammalian Expression, CRISPR
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
Gene/Insert
Gene/Insert namemAID-mClover-Neo
SpeciesH. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken)
Insert Size (bp)2875
Tag / Fusion Protein
mAID-mClover (C terminal on backbone)
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteBamHI (not destroyed)
5′ sequencing primerGTAAAACGACGGCCAGT
3′ sequencing primerGGAAACAGCTATGACCATG

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com