BioVector? NTCC? pM-ABE植物基因編輯質(zhì)粒BioVector? CRISPR載體

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

Vector backbonepCB301-2μ-HDV
Vector typePlant Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberLow Copy
Gene/Insert
Gene/Insert nameFull length TSWV M antigenome encoding ABE in place of viral GP
SpeciesSynthetic
MutationSee depositor comments below
Promoterduplicated cauliflower mosaic virus 35S promoter
Tag / Fusion Protein
3×flag (N terminal on insert)
Cloning Information
Cloning methodLigation Independent Cloning
5′ sequencing primer35S promoter/forward primer: CTATCCTTCGCAAGACCCTTC
3′ sequencing primerNos terminator/reverse primer: TCATCGCAAGACCGGCAA

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com