Catalog Number:

NTCC594202

Growth Properties
   :

adherent

Organism :

Homo sapiens (human)

Source :

Organ: mammary
   gland/breast

Cell type:epithelial-like

Disease: adenocarcinoma

Comments :

The
MDA-MB-231 human breast cancer cell line was established
   from tumor tissue from an untreated patient.

Propagation :

Complete growth medium:RPMI 1640 medium,
   90%; fetal bovine serum, 10%

Temperature:37.0℃,5%CO₂

Drug
   resistance:

The
   highest concentration of Taxol resistance is 30ng/ml.

Subculturing:

Protocol: Remove
   medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the
   solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the
   flask to sit at room temperature (or at 37℃) until the cells detach. Add fresh culture
   medium, aspirate and dispense into new culture flasks.

Subcultivation ratio:A
   subcultivation ratio of 1:2 to 1:4 is recommended

Medium renewal:Every 2 to
   3 days

Preservation :

Freeze medium:Complete growth
   medium, 95%; DMSO, 5%

Storage temperature:Liquid
   nitrogen vapor temperature