????S17-1 λpir E.coli strain

Organism:

Escherichia coli (Migula) Castellani and Chalmers

Designations:

S17-λpir

Genotype:

TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir.

Growth Conditions:

LB medium Temperature: 37.0°C

Applications:

transformation host

Comments:

E. coli S17-λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary).

BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心