pTKRED plasmid

pTKRED基因重組載體染色體整合質(zhì)粒低拷貝阿拉伯坦誘導(dǎo)型，含有repA101復(fù)制子，壯觀霉素抗性，I-SceI基因，ParaBAD阿拉伯糖誘導(dǎo)啟動子。

>pTKRED sequence 10726 bps

ctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcc

cgcacgatgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgca

tgcttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctg

gccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacgg

tggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagct

taagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgt

gcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtaccc

gattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcag

atttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcg

ctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaa

gccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatg

acgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt

ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtc

ggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaa

cgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaa

gaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacca

aaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaa

caaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgcc

atagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctc

catacccgtttttttgggaattcgagctctaaggaggttataaaaaatgcatcaaaaaaaccaggtaatg

aacctgggtccgaactctaaactgctgaaagaatacaaatcccagctgatcgaactgaacatcgaacagt

tcgaagcaggtatcggtctgatcctgggtgatgcttacatccgttctcgtgatgaaggtaaaacctactg

tatgcagttcgagtggaaaaacaaagcatacatggaccacgtatgtctgctgtacgatcagtgggtactg

tccccgccgcacaaaaaacaacgtgttaaccacctgggtaacctggtaatcacctggggcgcccagactt

tcaaacaccaagctttcaacaaactggctaacctgttcatcgttaacaacaaaaaaaccatcccgaacaa

cctggttgaaaactacctgaccccgatgtctctggcatactggttcatggatgatggtggtaaatgggat

tacaacaaaaactctaccaacaaatcgatcgtactgaacacccagtctttcactttcgaagaagtagaat

acctggttaagggtctgcgtaacaaattccaactgaactgttacgtaaaaatcaacaaaaacaaaccgat

catctacatcgattctatgtcttacctgatcttctacaacctgatcaaaccgtacctgatcccgcagatg

atgtacaaactgccgaacactatctcctccgaaactttcctgaaataagcatgcccgttccatacagaag

ctgggcgaacaaacgatgctcgccttccagaaaaccgaggatgcgaaccacttcatccggggtcagcacc

accggcaagcgccgcgacggccgaggtcttccgatctcctgaagccagggcagatccgtgcacagcacct

tgccgtagaagaacagcaaggccgccaatgcctgacgatgcgtggagaccgaaaccttgcgctcgttcgc

cagccaggacagaaatgcctcgacttcgctgctgcccaaggttgccgggtgacgcacaccgtggaaacgg

atgaaggcacgaacccagtggacataagcctgttcggttcgtaagctgtaatgcaagtagcgtatgcgct

cacgcaactggtccagaaccttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttg

ttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggt

cgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaaca

tcatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgcca

tctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagt

gatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgacc

ttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgca

cgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgac

attcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagag

aacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatt

tgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgta

gtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccg

actgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttgg

acaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgag

atcaccaaggtagtcggcaaataatgtctaacaattcgttcaagccgacgccgcttcgcggcgcggctta

actcaagcgttagatgcactaagcacataattgctcacagccaaactatcaggtcaagtctgcttttatt

atttttaagcgtgcataataagccctacacaaattgggagatatatcatgaaaggctggctttttcttgt

tatcgcaatagttggcgaagtaatcgcaacatccgcattaaaatctagcgagggctttactaagctcctg

cagagatctgaattccctagagagacgaaagtgattgcgcctacccggatattatcgtgaggatgcgtca

tcgccattgctccccaaatacaaaaccaatttcagccagtgcctcgtccattttttcgatgaactccggc

acgatctcgtcaaaactcgccatgtacttttcatcccgctcaatcacgacataatgcaggccttcacgct

tcatacgcgggtcatagttggcaaagtaccaggcattttttcgcgtcacccacatgctgtactgcacctg

ggccatgtaagctgactttatggcctcgaaaccaccgagccggaacttcatgaaatcccgggaggtaaac

gggcatttcagttcaaggccgttgccgtcactgcataaaccatcgggagagcaggcggtacgcatacttt

cgtcgcgatagatgatcggggattcagtaacattcacgccggaagtgaattcaaacagggttctggcgtc

gttctcgtactgttttccccaggccagtgctttagcgttaacttccggagccacaccggtgcaaacctca

gcaagcagggtgtggaagtaggacattttcatgtcaggccacttctttccggagcggggttttgctatca

cgttgtgaacttctgaagcggtgatgacgccgagccgtaatttgtgccacgcatcatccccctgttcgac

agctctcacatcgatcccggtacgctgcaggataatgtccggtgtcatgctgccaccttctgctctgcgg

ctttctgtttcaggaatccaagagcttttactgcttcggcctgtgtcagttctgacgatgcacgaatgtc

gcggcgaaatatctgggaacagagcggcaataagtcgtcatcccatgttttatccagggcgatcagcaga

gtgttaatctcctgcatggtttcatcgttaaccggagtgatgtcgcgttccggctgacgttctgcagtgt

atgcagtattttcgacaatgcgctcggcttcatccttgtcatagataccagcaaatccgaaggccagacg

ggcacactgaatcatggctttatgacgtaacatccgtttgggatgcgactgccacggccccgtgatttct

ctgccttcgcgagttttgaatggttcgcggcggcattcatccatccattcggtaacgcagatcggatgat

tacggtccttgcggtaaatccggcatgtacaggattcattgtcctgctcaaagtccatgccatcaaactg

ctggttttcattgatgatgcgggaccagccatcaacgcccaccaccggaacgatgccattctgcttatca

ggaaaggcgtaaatttctttcgtccacggattaaggccgtactggttggcaacgatcagtaatgcgatga

actgcgcatcgctggcatcacctttaaatgccgtctggcgaagagtggtgatcagttcctgtgggtcgac

agaatccatgccgacacgttcagccagcttcccagccagcgttgcgagtgcagtactcattcgttttata

cctctgaatcaatatcaacctggtggtgagcaatggtttcaaccatgtaccggatgtgttctgccatgcg

ctcctgaaactcaacatcgtcatcaaacgcacgggtaatggattttttgctggccccgtggcgttgcaaa

tgatcgatgcatagcgattcaaacaggtgctggggcaggcctttttccatgtcgtctgccagttctgcct

ctttctcttcacgggcgagctgctggtagtgacgcgcccagctctgagcctcaagacgatcctgaatgta

ataagcgttcatggctgaactcctgaaatagctgtgaaaatatcgcccgcgaaatgccgggctgattagg

aaaacaggaaagggggttagtgaatgcttttgcttgatctcagtttcagtattaatatccattttttata

agcgtcgactgtttcctgtgtgaaattgttatccgctcacaattccacacattatacgagccggaagcat

aaagtgtaaagcctggggtgcctaatgagtgagaattcggatctcgacgggatgttgattctgtcatggc

atatccttacaacttaaaaaagcaaaagggccgcagatgcgacccttgtgtatcaaacaagacgattaaa

aatcttcgttagtttctgctacgccttcgctatcatctacagagaaatccggcgttgagttcgggttgct

cagcagcaactcacgtactttcttctcgatctctttcgcggtttccgggttatctttcagccaggcagtc

gcattcgctttaccctgaccgatcttctcacctttgtagctgtaccacgcgcctgctttctcgatcagct

tctcttttacgcccaggtcaaccagttcgccgtagaagttgataccttcgccgtagaggatctggaattc

agcctgtttaaacggcgcagcgattttgttcttcaccactttcacgcgggtttcgctacccaccacgttt

tcgccctctttcaccgcgccgatacgacggatgtcgagacgaacagaggcgtagaatttcagcgcgttac

caccggtagtggtttccgggttaccgaacatcacaccaattttcatacggatctggttgatgaagatcag

cagcgtgttggactgcttcaggttacccgccagcttacgcatcgcctggctcatcatacgtgccgcaagg

cccatgtgagagtcgccgatttcgccttcgatttccgctttcggcgtcagtgccgccacggagtcaacga

cgataacgtctactgcgccagaacgcgccagggcgtcacagatttccagtgcctgctcgccggtgtccgg

ctgggagcacagcaggttgtcgatatcgacgcccagtttacgtgcgtagattgggtccagcgcgtgttca

gcatcgataaacgcacaggttttaccttcacgctgcgctgcggcgatcacctgcagcgtcagcgtggttt

taccggaagattccggtccgtagatttcgacgatacggcccatcggcagaccacctgccccaagcgcgat

atccagtgaaagcgaaccggtagagatggtttccacatccatggaacggtcttcacccaggcgcatgatg

gagcctttaccaaattgtttctcaatctggcccagtgctgccgccaacgctttctgtttgttttcgtcga

tagccatttttactcctgtcatgccgggtaataccggatagtcaatatgttctgttgaagcaattatact

gtatgctcatacagtatcaagtgttttgtagaaattgttgccacaaggtctgcaatgcatacgcagtagc

ctgacgacgcaccgcatcacggtcgccgctgaagcatcccgccgggtaatgccttcaccgcgggcagtgg

caaaagcaaaccagacggtgccgacaggcttctctagatccgcctacctttcacgagttgcgcagtttgt

ctgcaagactctatgagaagcagataagcgataagtttgctcaacatcttctcgggcataagtcggacac

catggcatcacagtatcgtgatgacagaggcagggagtgggacaaaattgaaatcaaataatgattttat

tttgactgatagtgacctgttcgttgcaacaaattgataagcaatgccaagcttggcactggctgatcag

ctagctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgc

ggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctg

attgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcga

aaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccacta

ccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatc

gttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggac

atggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagc

cagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaa

tgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtc

tggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggt

catccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttaca

ggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagattta

atcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgact

gtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttt

ttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccg

gcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggc

gctatcatgccataccgcgaaaggttttgcaccattcgatgctagcccatgggtatggacagttttccct

ttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaag

agccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgt

gagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactg

gtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctg

atgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc

catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaat

ttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaact

catggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtg

agttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagac

ttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaa

aaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaac

caaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagca

ttttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttg

tagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaa

gtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtct

aggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcctttgag

ttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaa

ttccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaa

taaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtatt

acaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtag

caccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatattccttttgtctccgaccatca

ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatggggg

taaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtc

acgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagt

tcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatg

cacccagtaaggcagcggtatcatcaacggggtctgacgctcagtggaacgaaaactcacgttaagggat

tttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatca

atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcag

cgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggaggg

cttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca

ataaaccagccagccg