pBACe3.6 BAC cloning plasmid

pBACe3.6 maintained the wildtype loxP site, added an additionalmutant loxP511 site, a site for the intron encoded nuclease PI-SceI and theTn7att site.

The presence of this pUC plasmid serves dual functions: high copynumber of the vector for preparing large quantities and appropriate disruptionof the sacBII gene to increase viability of the vector containing strain. In addition to the BamHI site, 5 additional sites can be used for preparing BAClibraries: SacI, SacII, MluI, EcoRI, and AvaIII.  The EcoRI site isparticularly important in view of the use of this site in the RARE cleavageprocedure, thus opening the possibility for selective cloning of similarfragments from different DNA donors.

pBACe3.6 clones have chloramphenicol antibiotic resistance. Clones should be grown in LB containing 12.5 ug chloramphenicol/ml.

Reference: Frengen, E. et al. (1999) AModular, Positive Selection Bacterial Artificial Chromosome Vector withMultiple Cloning Sites. Genomics 58: 250-253. Reprints availiable upon request.

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