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p426-SNR52p-gRNA.CAN1.Y-SUP4t
描述：
酵母CRISPR-Cas9系統(tǒng)質(zhì)粒載體，URA3篩選標(biāo)記，p426載體骨架，2u ori酵母復(fù)制起始位點，可插入guideRNA序列。
Encodes a gRNA that targets Can1.Y in yeast.
gRNA target sequence GATACGTTCTCTATGGAGGA
BACKBONE
- Vector backbonep426
Backbone size w/o insert (bp)5886
Total vector size (bp)6274
- Vector typeYeast Expression, CRISPR
- Selectable markersURA3
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
GENE/INSERT
- Gene/Insert nameCAN1.y gRNA
- SpeciesS. cerevisiae (budding yeast)
- Insert Size (bp)488
- Entrez GeneCAN1(a.k.a.YEL063C)
PromoterSNR52
CLONING INFORMATION
Cloning methodUnknown
5′ sequencing primerT3 promoter
3′ sequencing primerT7 promoter
Map圖譜：
Sequence序列：
>p426-SNR52p-gRNA.CAN1.Y-SUP4t sequence 6274 bps
gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagtatga
tccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaa
cagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtac
tagactacctttcatcctacataaatagacgcatataagtacgcatttaagcataaacacgcactatgcc
gttcttctcatgtatatatatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatg
tgcgcagctcgcgttgcattttcggaagcgctcgttttcggaaacgctttgaagttcctattccgaagtt
cctattctctagaaagtataggaacttcagagcgcttttgaaaaccaaaagcgctctgaagacgcacttt
caaaaaaccaaaaacgcaccggactgtaacgagctactaaaatattgcgaataccgcttccacaaacatt
gctcaaaagtatctctttgctatatatctctgtgctatatccctatataacctacccatccacctttcgc
tccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctttagacaa
aaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaaacatttcctatacg
tagtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatc
actttctgttcacaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaa
aaaatgcacccgcagcttcgctagtaatcagtaaacgcgggaagtggagtcaggctttttttatggaaga
gaaaatagacaccaaagtagccttcttctaaccttaacggacctacagtgcaaaaagttatcaagagact
gcattatagagcgcacaaaggagaaaaaaagtaatctaagatgctttgttagaaaaatagcgctctcggg
atgcatttttgtagaacaaaaaagaagtatagattctttgttggtaaaatagcgctctcgcgttgcattt
ctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgtttt
acaaaaatgaagcacagattcttcgttggtaaaatagcgctttcgcgttgcatttctgttctgtaaaaat
gcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcaca
gatgcttcgttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaata
cattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaaga
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgc
tcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaa
ctggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactt
ttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcat
acactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgaca
gtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacga
tcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttg
ggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaaca
acgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatgg
aggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatc
tggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatc
gtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtg
cctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaact
tcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgt
gagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttc
tgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaaga
gctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtg
tagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgt
taccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacc
gaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggt
atccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatct
ttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgg
agcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcaca
tgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgc
tcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaa
ccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacactttatgct
tccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgat
tacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagcttctttgaaaagataatgtat
gattatgctttcactcatatttatacagaaacttgatgttttctttcgagtatatacaaggtgattacat
gtacgtttgaagtacaactctagattttgtagtgccctcttgggctagcggtaaaggtgcgcattttttc
acaccctacaatgttctgttcaaaagattttggtcaaacgctgtagaagtgaaagttggtgcgcatgttt
cggcgttcgaaacttctccgcagtgaaagataaatgatcGATACGTTCTCTATGGAGGAGTTTTAGAGCT
AGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGGTGCTT
TTTTTGTTTTTTATGTCTtcgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccaca
tccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagtta
tgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatg
taacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggccggtac
ccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactggga
aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa
gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgta
gcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagc
gcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaat
cgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtg
atggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctt
taatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataa
gggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaatttta
acaaaatattaacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacac
cgcatagggtaataactgatataattaaattgaagctctaatttgtgagtttagtatacatgcatttact
tataatacagttttttagttttgctggccgcatcttctcaaatatgcttcccagcctgcttttctgtaac
gttcaccctctaccttagcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcc
tgtagagaccacatcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaaacccaca
ccgggtgtcataatcaaccaatcgtaaccttcatctcttccacccatgtctctttgagcaataaagccga
taacaaaatctttgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagcc
cttgcatgacaattctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgcttc
aaaccgctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctattctgt
atacacccgcagagtactgcaatttgactgtattaccaatgtcagcaaattttctgtcttcgaagagtaa
aaaattgtacttggcggataatgcctttagcggcttaactgtgccctccatggaaaaatcagtcaagata
tccacatgtgtttttagtaaacaaattttgggacctaatgcttcaactaactccagtaattccttggtgg
tacgaacatccaatgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggcagcaac
aggactaggatgagtagcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctgcag
gtttttgttctgtgcagttgggttaagaatactgggcaatttcatgtttcttcaacactacatatgcgta
tatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaa
atttcaaGgaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattgaaaagctgtggta
tggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccg
ctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggag
ctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga
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