The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.

The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.

pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).

序列Sequence：

AGATCTAGTCTTATAACTATACTGACAATAGAAACATTAACAAATCTAAAACAGTCTTAATTCTATCTTG

AGAAAGTATTGGTAATAATATTATTGTCGATAACGCGAGCATAATAAACGGCTCTGATTAAATTCTGAAG

TTTGTTAGATACAATGATTTCGTTCGAAGGAACTACAAAATAAATTATAAGGAGGCACTCACCATGGGTA

CTGCAGGCATGCGGTACCACTAGTTCTAGAGAGCTCAAGCTTTCTTTGAACCAAAATTAGAAAACCAAGG

CTTGAAACGTTCAATTGAAATGGCAATTAAACAAATTACAGCACGTGTTGCTTTGATTGATAGCCAAAAA

GCAGCAGTTGATAAAGCAATTACTGATATTGCTGAAAAATTGTAATTTATAAATAAAAATCACCTTTTAG

AGGTGGTTTTTTTATTTATAAATTATTCGTTTGATTTCGCTTTCGATAGAACAATCAAATCGTTTCTGAG

ACGTTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGC

CCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAA

ATAATAAGCGCAGCGTCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTTTGAGGGAATGAAATTCCCT

CATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAAT

TTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTG

CCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTGAGGGGTTTTTCAATC

GCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTTTTGTTT

TTATGATTACAAAGTGATACACTAATTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAG

AATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACA

AAAAGATTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAA

AAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATT

ATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAA

TGATTGGAAAGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAA

AAAAAAGATAAAGATACATGGAATAGTAGTGATGTTATACGAAATGGAAAGCACTATAAAAAACCACACT

ATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATT

GGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCAT

GAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATT

TTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTTTTAGATAT

AGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTT

GGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTG

AGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAA

ATGACAAACAAAGAAAAAGAGTTATTTGCTGAAAATGAGGAATTAAAAAAAGAAATTAAGGACTTAAAAG

AGCGTATTGAAAGATACAGAGAAATGGAAGTTGAATTAAGTACAACAATAGATTTATTGAGAGGAGGGAT

TATTGAATAAATAAAAGCCCCCCTGACGAAAGTCGACGGCAATAGTTACCCTTATTATCAAGATAAGAAA

GAAAAGGATTTTTCGCTACGCTCAAATCCTTTAAAAAAACACAAAAGACCACATTTTTTAATGTGGTCTT

TTATTCTTCAACTAAAGCACCCATTAGTTCAACAAACGAAAATTGGATAAAGTGGGATATTTTTAAAATA

TATATTTATGTTACAGTAATATTGACTTTTAAAAAAGGATTGATTCTAATGAAGAAAGCAGACAAGTAAG

CCTCCTAAATTCACTTTAGATAAAAATTTAGGAGGCATATCAAATGAACTTTAATAAAATTGATTTAGAC

AATTGGAAGAGAAAAGAGATATTTAATCATTATTTGAACCAACAAACGACTTTTAGTATAACCACAGAAA

TTGATATTAGTGTTTTATACCGAAACATAAAACAAGAAGGATATAAATTTTACCCTGCATTTATTTTCTT

AGTGACAAGGGTGATAAACTCAAATACAGCTTTTAGAACTGGTTACAATAGCGACGGAGAGTTAGGTTAT

TGGGATAAGTTAGAGCCACTTTATACAATTTTTGATGGTGTATCTAAAACATTCTCTGGTATTTGGACTC

CTGTAAAGAATGACTTCAAAGAGTTTTATGATTTATACCTTTCTGATGTAGAGAAATATAATGGTTCGGG

GAAATTGTTTCCCAAAACACCTATACCTGAAAATGCTTTTTCTCTTTCTATTATTCCTTGGACTTCATTT

ACTGGGTTTAACTTAAATATCAATAATAATAGTAATTACCTTCTACCCATTATTACAGCAGGAAAATTCA

TTAATAAAGGTAATTCAATATATTTACCGCTATCTTTACAGGTACATCATTCTGTTTGTGATGGTTATCA

TGCTGGATTGTTTATGAACTCTATTCAGGAATTGTCAGATAGGCCTAATGACTGGCTTTTATAATATGAG

ATAATGCCGACTGTACTTTTTACAGTCGGTTTTCTAATGTCACTAACCTGCCCCGTTAGTTGAAGAAGGT

TTTTATATTACAGCTCC

質(zhì)粒載體菌株細(xì)胞株基因庫(kù)-Biovector Inc.典型培養(yǎng)物保藏中心NTCC，可提供數(shù)萬種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純化、病毒包裝、基因沉默RNAi等服務(wù)。作為美國(guó)質(zhì)粒載體菌株細(xì)胞株基因保藏中心分庫(kù)及唯一辦事處，Biovector Inc.可為您提供便捷一站式的產(chǎn)品進(jìn)口服務(wù)，到貨快捷，步驟簡(jiǎn)便。

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資源涵蓋克隆載體、真核表達(dá)載體、原核表達(dá)載體、病毒載體、信號(hào)通路報(bào)告載體、亞細(xì)胞定位載體、熒光蛋白載體、昆蟲細(xì)胞表達(dá)載體、啟動(dòng)子載體、誘導(dǎo)調(diào)控載體等十多種類型上萬個(gè)品種。
我們擁有目前最大的cDNA克隆庫(kù)、覆蓋30,000個(gè)人全長(zhǎng)基因的TrueClone cDNA克隆和超過25,000個(gè)TrueORF cDNA克隆。利用TrueORF cDNA克隆，我們開發(fā)了大量的全長(zhǎng)人重組蛋白產(chǎn)品（哺乳動(dòng)物細(xì)胞表達(dá)），可以進(jìn)行蛋白功能的研究。

標(biāo)簽：以下資源由Biovector Inc. 質(zhì)粒載體菌株細(xì)胞株基因保藏中心獨(dú)家供應(yīng)并發(fā)布，侵權(quán)必究。Biovector,Inc質(zhì)粒載體菌株細(xì)胞株基因保藏中心列表網(wǎng)址http://biovector.blog.163.com 質(zhì)粒載體索取報(bào)價(jià)價(jià)格優(yōu)惠售價(jià)出售圖譜序列酶切位點(diǎn)轉(zhuǎn)化構(gòu)建求購(gòu)購(gòu)買訂購(gòu) sequence map plasmid vector gene strain order ship 抗性 resistance restriction site

Taq,Pfu高表達(dá)載體菌株；KOD,T4連接酶等高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶，Tth反轉(zhuǎn)錄酶MMLV，Klentaq PCR酶，Cre酶，RNA inhibitor抑制酶，DL2000質(zhì)粒

Gateway入門載體（gentamicin抗性）pDONR207，植物表達(dá)載體pJIT163-hGFP，pJIT166，植物雙分子熒光互補(bǔ)(bimolecular fluorescence complementation，BiFC)載體pCCFP-X,pX-CCFP,pX-NYFP,pNYFP-X；

乳酸菌表達(dá)載體系列

隱藏編輯

乳酸菌表達(dá)載體pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523，pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii

枯草芽孢桿菌表達(dá)載體系列

隱藏編輯

pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01， 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33

腺病毒/慢病毒逆病毒載體系列

隱藏編輯

腺病毒系統(tǒng)（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)（Stratagene）： pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS， 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝：二質(zhì)粒包裝系統(tǒng)一： psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二： pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率： pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng)： PLP1 PLP2 PLP/VSVG

原核表達(dá)載體系列

隱藏編輯

pQE系列pQE30,31,32,40,60,61,62, pGEX系列(含GST標(biāo)簽)，pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光),pET系列pET-GST,pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-),pINIIIompA,九載體原核表達(dá)試劑盒(高效、可溶、分泌、標(biāo)簽)pLLP-OmpA、pLLP-STII、pMBP-P、pMBP-C,pTrc-CKS,pET-GST、pET-Trx、pET-His,pET-DsbA,大腸桿菌冷激質(zhì)粒： pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大腸桿菌分子伴侶： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞： DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a（pir） Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

畢赤/釀酒酵母表達(dá)載體系列

隱藏編輯

釀酒酵母表達(dá)載體pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,pRS41H,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109；質(zhì)粒pGADT7,pGBKT7；對(duì)照質(zhì)粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套畢赤酵母Pichiapink,畢赤酵母宿主X33，KM71，KM71H，GS115,

植物表達(dá)/RNAi載體農(nóng)桿菌系列

隱藏編輯

pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體(隨機(jī)突變體庫(kù))pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1

RNAi基因沉默干擾載體系列

隱藏編輯

pSilencer1.0，pSilencer 2.1-U6 hygro， pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo， pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體（oligoengine） pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q， RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide） RNAi慢病毒載體: pLKO.1

基因cDNA文庫(kù)ORF表達(dá)載體系列

隱藏編輯

擁有目前最大的cDNA克隆庫(kù)、覆蓋10萬人、鼠全長(zhǎng)基因的cDNA克隆和超過25,000個(gè)ORF克隆表達(dá)載體。藥物轉(zhuǎn)運(yùn)體基因蛋白表達(dá)細(xì)胞株：肝臟 P-gp/MDR1 OAT2 OAT7 1-Oct OATP8 (OATP1B3) OATP-B (OATP2B1) OATP2（OATP1B1) 腎臟 P-gp/MDR1 OAT1 OAT2 OAT3 OAT4 2-Oct URAT1 PEPT1 PEPT2 OCTN1 OCTN2 小腸 P-gp/MDR1 1-Oct PEPT1 OATP 腦血屏障 P-gp/MDR1 BCRP OATP-A (OATP1A2) OATP-B (OATP2B1)

哺乳動(dòng)物表達(dá),GS系統(tǒng)

隱藏編輯

谷氨酰胺合成酶加壓篩選表達(dá)系統(tǒng)GS expression system vectors pEE12.4, pEE6.4, 配套CHOK1SV細(xì)胞株，GS表達(dá)載體pGP3, pGP3Due, pGP3Con, 配套cGMP banking record cell line，DHFR篩選表達(dá)系統(tǒng)載體pSV2-DHFR，pCI-DHFR, pcDNA4-DHFR, pGP2, pGP2Due, pGP2Con, 疫苗/抗體表達(dá)載體pCEP4,細(xì)胞高表達(dá)載體pTT5,配套細(xì)胞株HEK293E, 哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1(-)/myc-His A ，pcDNA6-Myc/His B， pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信號(hào)通路報(bào)告載體:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系統(tǒng)（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR

熱門日志

隱藏

綠色熒光pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1，pAcGFP1-1, pEGFP-Actin,  pIRES2-EGFP,pCMV/myc/Nuc,pCMV/myc/Nuc-GFP， pLEGFP-C1，pLEGFP-N1，青色熒光pECFP-N1，pECFP-C1，pECFP-ER由Biovector公司現(xiàn)貨供應(yīng)

W3110 strain菌株現(xiàn)貨供應(yīng)-Biovector質(zhì)粒菌株保藏中心

Biovector公司pCDH-EF1-MCS-T2A-Puro pLVTHM pLOX-CW-CRE pP-αSUMO3 SUMO protease 2菌株 PiggyBac轉(zhuǎn)座子系統(tǒng)Super PiggyBac Transposase (PB200PA-1)  PiggyBac Dual promoter (PB513B-1)  PiggyBac 3’LTR (pMD18T pCDH-EF1-MCS-T2A-Puro pBI CMV1 BL21gold(DE3) plysS菌株 pET-SUMO

Biovector公司pCDH-EF1-MCS-T2A-Puro  pLVTHM  pLOX-CW-CRE  pP-αSUMO3  SUMO protease 2菌株 pCDH-EF1-MCS-T2A-Puro  pBI CMV1  BL21gold(DE3) plysS菌株 pET-SUMO

pIRES2-DsRed， pmCherry-N1，pmCherry-C1，黃色熒光pEYFP-C1, pEYFP-N1, pEYFP-Golgi,  pEYFP-Mem,  pEYFP-ER , pEYFP actin，pEYFP tub由Biovector公司現(xiàn)貨供應(yīng)

Biovector公司pLOX-Ttag-iresTK  pLOX-TERT-iresTK  pLOX-CWBmi1  pFP93  pCDF1-MCS2-EF1-copGFP  pLentG-KOSM  TetO-FUW-OSKM  FUW-M2rtTA pCDH-CMV-MCS-EF1-GFP+Puro (pCD513B-1)  pWPXL  pCDH-CMV-MCS-EF1-copGFP

Biovector公司供應(yīng)ExAssist interference-resistant helper phage EZ10 FOPFlash   XL1-Blue MRF’ XL2-Blue MRF’ XLOLR Y187 Y2H Gold Yeast strain FT-T FT-T Simple FUGW FUW-M2rtTA FUW-tetO-hKLF4 FUW-tetO-hMYC FUW-tetO-hOCT4 FUW-tetO-hSOX2 GM2163 GS115 HA-mPB HB2151 H

Biovector公司pHSE-Luc  pGRE-Luc  pCRE-Luc  pAP1(PMA)-TA-Luc  pCaspase3-sensor  pIκB-EGFP  PiggyBac轉(zhuǎn)座子 系統(tǒng)Super PiggyBac Transposase (PB200PA-1)  PiggyBac Dual promoter (PB513B-1)  PiggyBac 3’LTR (pMD18T vector)  PiggyBac 5’LTR (pMD18T vector)  HA-mPB

廣宿主載體系列假單胞菌鏈球菌表達(dá)載體

隱藏編輯

pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1

基因重組敲除/自殺質(zhì)粒載體系列

隱藏編輯

pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽(yáng)性菌基因敲除)，pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253，PL451，PL452，PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374

熒光蛋白表達(dá)載體

隱藏編輯

pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen，pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1，pLEGFP-N1，pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動(dòng)子增強(qiáng)子報(bào)告載體（Promega，帶有Luc熒光報(bào)告基因）： pGL3-basic，pGL3-control，pGL3-enhancer，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40, 信號(hào)通路報(bào)告載體（Clontech）： pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP， pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc 信號(hào)通路報(bào)告載體（Promega）： pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75 信號(hào)通路報(bào)告載體（Stratagene）： p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc， pFA2-Elk1，pFC-MEKK，pFR-luc

昆蟲/桿狀病毒表達(dá)載體

隱藏編輯

pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His

最新日志

隱藏

pX330  pX335  hCas9  gRNA_CloningVector  pX260 pX334  hCas9_D10A  MLM3613  DR274  pMD2.G Golden Gate TALEN Kit 2.0  TALE Toolbox  TALEN Accessory Pack  LIC TAL Effector Assembly Kit  CCSB-Broad Human Kinase ORF Collection由Biovector Inc.現(xiàn)貨供應(yīng)

EL350 EL350 (galk+) EL350 (galk-) WB800 ATCC 13032 BL21（DE3） 2T1R(CCDB) JM109 DH10B DH-5α 由質(zhì)粒載體菌株細(xì)胞株基因保藏中心現(xiàn)貨供應(yīng)

pMXS-puro pMXS-puro-luciferase pMXS-puro-H2B-eGFP plko.1-IRES-GFP plko.1-EGFP plko.1-H2B-GFP plko.1-linker plko.1-CMV plko.1-trc Lactobacillus sake CGMCC 1.2244 CGMCC1.1215 ATCC 13032 WB800 2T1R(CCDB) BL21（DE3） EL350 EL350 (galk+) EL350 (galk-)由普如汀公司現(xiàn)貨供應(yīng)

pCDNA3.1-Home-ERK2 pcDNA3.1 pWB980 pET-22b-sit pBV220 pBV220-gam-bet-txo pXMJ-19 pENTR gus pEF-EntrB pENTR-3c-IRES-GFP pEntr-3c-Home-ERK1 pMXS-IRES-GFP pDEST 8 pDEST 27 pcDNA DEST-40 pcDNA DEST-47 pcDNA DEST-53 plko.1-RFPuro plko.1-d2eGFP由質(zhì)粒菌株庫(kù)現(xiàn)貨供應(yīng)

pCAG-Cre-GFP pCAG-Cre pCAG-Cre-ERT2 pGIPZ-akt1 pFD pBFP-IRES-puro pLC3-CFP pLamp1-cherry red pLamp1-YFP p3XFlag-CMV-7.1-Homo-MKL1 pSG5-Homo-SRCIa pEGFP-IRES-neo pEYFP-N1 pEGFP-C3 pRK5-Home-smad3 pRK5-Home-smad2 pCDNA3.1-Home-ER α 由質(zhì)粒載體菌株細(xì)胞株基因保藏中心現(xiàn)貨供應(yīng)

pCDNA3.1-Mouse-MRTF-A pCDNA3.1-Mouse-myocardin pCDNA3.1-Mouse-stat3F pEF1 pBS-MDa pPK2 pCMV-GAG-pol pDEST 12.2 pTranslucent pSJ4 pANGH3 pGL4.74hRluc/TK ptTs-NEO pQC-tTS-IN pSIREN-retorQ pSinoa pGL3-Basic pAmpho pFD-NTC phelp pECO p10A1由質(zhì)粒菌株庫(kù)現(xiàn)貨供應(yīng)

BL21 SI BL21 Star (DE3) C600 DH5α GI698 GI724 HB2151 JF1125 JM105 JM109 JM109(DE3) K802 LMG194 YK537 GS115 GS115β-Gal GS115 HisA菌株由Biovector公司現(xiàn)貨供應(yīng)

W3110 strain菌株現(xiàn)貨供應(yīng)-Biovector質(zhì)粒菌株保藏中心

BiFC雙分子熒光質(zhì)粒

隱藏編輯

pBiFC-VC155，pBiFC-VN173，pBiFC-bFosVC155，pBiFC-bFos(deltaZIP)VC155，pBiFC-bjun-VN173，pCE-BiFC-VN173，pCE-BiFC-VC155

真菌/黑、黃曲霉表達(dá)載體

隱藏編輯

pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等

酵母單雜/雙雜/三雜交載體系列

隱藏編輯

雙雜交系統(tǒng)pGBKT7，pGADT7，pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam，配套釀酒酵母AH109 酵母單雜交系統(tǒng)（Clontech）:pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System（Stratagene）: pSos, pSos MAFB, pMyr

噬菌體展示系統(tǒng)

隱藏編輯

1.噬菌體展示抗體scFv 質(zhì)粒：pCANTAB5E 宿主菌：TG1 輔助噬菌體：M13K07 可溶表達(dá)菌：HB2151 2.噬菌體展示抗體Fab 質(zhì)粒：pcomb3 宿主菌：XL1-Blue 輔助噬菌體：VCSM13千余種細(xì)胞株：CHOK1-SV，HEK293T,FT,HEK293E,HEK293-6E,Sf9,肺癌細(xì)胞，乳腺癌細(xì)胞，猴腎細(xì)胞，前列腺癌細(xì)胞，卵巢細(xì)胞，肝癌細(xì)胞，食管癌細(xì)胞