pCLR2068哺乳動物基因編輯載體斑馬魚基因編輯質(zhì)粒

BioVector? pCLR2068 plasmidFor expression of a ZFN targeted to a site with a 5 or 6 bp spacer and “+” KKR heterodimeric FokI domain. (Doyon et. al,Nat Biotech 2011) in mammalian cells or zebrafish as described by Moore et al., PLoS One 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22655075) and Cade et al., Nucleic Acids Res 2012 (https://www.ncbi.nlm.nih.gov/pubmed/22684503)BACKBONEVector backbone BioVector? pMLM290Backbone size w/o insert (bp)5725Vector typeMammalian Expression ; Zebrafish expressionGROWTH IN BACTERIABacterial Resistance(s)Ampicillin, 100 μg/mLGrowth Temperature37°CGrowth Strain(s)DH5alphaCopy numberHigh CopyGENE/INSERTGene/Insert nameFokI endonucleaseMutationContains H537R point mutation in FokI domain, as well as E490K and I538K present in the vector backboneTag / Fusion ProteinFLAG (N terminal on backbone)CLONING INFORMATIONCloning methodRestriction Enzyme5′ cloning siteXbaI (not destroyed)3′ cloning siteBamHI (not destroyed)5′ sequencing primerCMV-F

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