pICH-chl-roGFP2-ORP1 plasmid vector植物過(guò)氧化物熒光檢測(cè)質(zhì)粒載體

Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.
While versions of this fusion may exist elswhere, ours was synthesized de novo.
BACKBONE
Vector backbonepICH86988
(Search Vector Database)
Backbone manufacturerGolden Gate Addgene number #48076
Backbone size w/o insert (bp)9078
Total vector size (bp)9797
Modifications to backboneAdded in during cloning: The level 0 chloroplast transiit peptide coding sequence from pICH78133 (Addgene #50292).
Vector typePlant Expression ; Binary Agrobacterium Ti plasmid
Selectable markerskanamycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namechl-roGFP2-ORP1
SpeciesSynthetic
Insert Size (bp)1488
PromoterCaMV 35S promoter
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBsa1 (destroyed during cloning)
3′ cloning siteBsa1 (destroyed during cloning)

Supplier來(lái)源:BioVector NTCC Inc.
TEL電話:400-800-2947
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