MoClo-YTK Moclo Yeast toolkit

This collection contains a set of 96 standardized and characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. The toolkit was deposited by John Dueber’s lab and contains characterized promoters, terminators, peptide tags, copy number machinery, and genome-editing tools. The hierarchical assembly scheme is based on the Golden Gate cloning technique, but it is also designed to be compatible with recombination based cloning such as yeast in vivo assembly, ligation independent cloning (SLIC) or Gibson Assembly. The design specifications of this collection also make constructs BioBrick-compatible. Use of this workflow allows for construction of multi-gene plasmids from PCR templates in three days.

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