Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2 plasmid vector細(xì)胞周期熒光報(bào)告質(zhì)粒載體

Fluorescent reporter vector to visualize all of cell cycle phases.

BACKBONE
Vector backbonePB510B-1
(Search Vector Database)
Backbone manufacturerSystem Biosciences
Vector typeMammalian Expression, Bacterial Expression
Selectable markersPuromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberUnknown
GENE/INSERT 1
Gene/Insert nameiRFP
SpeciesSynthetic
PromoterCMV
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodRestriction Enzyme
5′ cloning siteXbaI (unknown if destroyed)
3′ cloning siteXbaI (unknown if destroyed)
5′ sequencing primercgcaaatgggcggtaggcgtg
3′ sequencing primerUnknown
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert namehCdt1(1/100)Cy(-)
Alt nameCDT1
SpeciesSynthetic
Entrez GeneCdt1 (a.k.a. 2610318F11Rik, DUP, Ris2)
PromoterCMV
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodLigation Independent Cloning
5′ sequencing primercgcaaatgggcggtaggcgtg
3′ sequencing primerUnknown
(Common Sequencing Primers)
GENE/INSERT 3
Gene/Insert nameTagBFP2
SpeciesSynthetic
PromoterCMV
CLONING INFORMATION FOR GENE/INSERT 3
Cloning methodRestriction Enzyme
5′ cloning siteAvrII (unknown if destroyed)
3′ cloning siteNdeI (unknown if destroyed)
5′ sequencing primerGAAGAAAACACTCGGCTGGGA
3′ sequencing primertacaaaggcattaaagcagcgta
(Common Sequencing Primers)
GENE/INSERT 4
Gene/Insert namehGeminin(1/110)
Alt nameGMNN
SpeciesSynthetic
GenBank IDNM_001251989.2
PromoterCMV
CLONING INFORMATION FOR GENE/INSERT 4
Cloning methodRestriction Enzyme
5′ cloning siteNdeI (unknown if destroyed)
3′ cloning siteNdeI (unknown if destroyed)
5′ sequencing primerGAAGAAAACACTCGGCTGGGA
3′ sequencing primerAAACAACAGATGGCTGGCAAC

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
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