PB-UniSAM plasmid vector哺乳動(dòng)物基因編輯質(zhì)粒載體

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

BACKBONE
Vector backbonePiggy Bac
(Search Vector Database)
Backbone size w/o insert (bp)3961
Vector typeMammalian Expression, CRISPR, Synthetic Biology ; dCAS9-SAM activation
Selectable markersmCherry
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberLow Copy
GENE/INSERT
Gene/Insert nameUniSAM-mCherry + U6-gRNA2.0
Alt nameCas9VP64-MS2p65HSF1-mCherry + gRNA(2xMS2Loops)
Insert Size (bp)8388
MutationBbsI sites in CDS were ablated by consensus mutation codon optimised for H.sapiens
PromoterEF1a
CLONING INFORMATION
Cloning methodGibson Cloning
5′ sequencing primerEXT_PB_RV--GGCACATATCAATATTATGCTCTCG
3′ sequencing primerEXT_PB_FW--GCAAAGCGATGACGAGCTTG

Supplier來源:BioVector NTCC Inc.
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