MDCC-MSB-1 cell line雞淋巴瘤細胞株

NTCC? General information
Organism Chicken
Disease Marek disease
Synonyms MDCC MSB1, MDCC-MSB-1, MSB-1, MSB1
Characteristics
Morphology Round cells
Cell type Lymphoblast
Growth properties Suspension

Handling
Culture Medium NTCC? RPMI 1640
Medium supplements 10% FBS, 2.5 mM L-glutamine
Passaging solution The utilization of a passaging solution is not necessary when passaging cells that are cultured in suspension. The appropriate procedure is to dilute the cells in accordance with the indicated guidelines.
Doubling time 10 hours
Subculturing Establish new cultures at 3 x 10^5 viable cells/ml. Maintain the cell density between 1 x 10^5 and 1 x 10^6 cells/ml by transferring an appropriate amount of cell suspension into a new cell culture flask refilled with fresh cell culture medium.
Seeding density 1 x 10^6 cells/ml
Fluid renewal 2 to 3 times per week
Freezing recovery After thawing, allow the cells to recover from the freezing process for at least 24 hours.

Supplier來源:BioVector NTCC Inc.
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