AAEL005635-Cas9 plasmid vector昆蟲基因編輯質(zhì)粒載體

PURPOSE
The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

BACKBONE
Vector backbonePiggyBac
(Search Vector Database)
Backbone size w/o insert (bp)5246
Total vector size (bp)15788
Vector typeInsect Expression, CRISPR
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT 1
Gene/Insert nameAAEL005635-hspcas9-GFP
SpeciesAedes aegypti, Streptococcus pyogenes
Insert Size (bp)8768
PromoterAAEL005635
Tag / Fusion Protein
GFP (C terminal on insert)
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodGibson Cloning
5′ sequencing primerCGACGGTCACGGCGGGCATGTCGACGCGGCCGCATGTGAATCAACATTAGGTTGTCATGA
3′ sequencing primerAAAAGTTGGTGGTGGGGAGGCCACCGAGTATGGGCGCGCCCCGGCCGTTAACTCGAATCG
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert nameOpie2-dsRed
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)926
PromoterOpie2
Tag / Fusion Protein
dsRED (C terminal on insert)
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodGibson Cloning
5′ sequencing primerGGATAGCGATTCGAGTTAACGGCCGGGgcgcgcccatactcggtggcctccccacca
3′ sequencing primerAACATTGTCAGATCCGAGATCGGCCGGCCTAGAAGCTTTAAGATACATTGATGAGTTTGGAC

Supplier來源:BioVector NTCC Inc.
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