PU6::unc-119_sgRNA C.elegans gene editing plasmid線蟲基因編輯質(zhì)粒載體

gRNA target sequence GAATTTTCTGAAATTAAAGA

Vector backbone pUC57
Backbone size w/o insert (bp)2641
Total vector size (bp)3481
Vector typeCRISPR
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameunc-119 targeting sgRNA
Alt nameunc-119
SpeciesSynthetic
Insert Size (bp)840
Entrez Geneunc-119 (a.k.a. CELE_M142.1)
PromoterC. elegans U6 snRNA pol III promoter
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteEcoRI (not destroyed)
3′ cloning siteHindIII (not destroyed)
5′ sequencing primerM13 (-40) forward
3′ sequencing primerM13 (-48) reverse

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網(wǎng)址: http://www.nedfriskphoto.com