R1000 A.rhizogenes strain/Competent cells發(fā)根農(nóng)桿菌菌株/感受態(tài)細(xì)胞

The initial strains, R1000 and R1500, from which R1600 and R1601 were isolated (Figure 1), could not induce hairy roots on hybrid poplar (Populus trichocarpa × deltoides). Enhancing the virulence of R1000 and R1500 by adding the pTVK291 cosmid that carries part of the vir region of pTiBO342 (conferring the supervirulent phenotype to the A. tumefaciens strain A348) permitted the generation of hybrid poplar hairy roots and the subsequent regeneration of shoots from those hairy roots [70]. A similar modification was carried out for A. tumefaciens, i.e., the stable transformation rate of tomato (Solanum lycopersicum) with the resulting strain was increased 3.6-fold compared with that for the original GV2260 strain

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