TA1535/pSK1002 BioVector’s Salmonella typhimurium strain腸道沙門氏菌環(huán)境水質(zhì)檢測AMES檢測用菌-BioVector NTCC Inc.
- 價 格：￥49865
- 貨 號：Biovector502709
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作微信:1843439339 (QQ同號)
手機：18901268599
地址：北京
- 已注冊
The multicopy plasmid BioVectorTM pSK1002, a fusion product of genes of theumu operon (which was derived
from E. coli K12 AB1157) with the vector pMC1403 (a pBR322 derivative), carries a umuC'-lacZ gene fusion
product. The umu operon is regulated by the SOS genes recA (protease) and lexA (repressor) and consists of a
promoter, operator,umuD and umuC genes. The vector carries the lacZ and lacY genes, the ampicillin resistance
gene and ori. The strain BioVectorTM TA1535 (AMES test strain) is uvrB (deficient in excision repair), rfa (incomplete cell wall
causing good permeability) and does not possess a chromosomallacZ gene. pSK1002 makes the strain
selectable, and the umuCgene product (UmuC protein) functions as a mutator protein. The umutest is a
short-term genotoxicity assay for the detection of mutagenic potentials of chemicals etc.
Mol. weight:
15 kb
Marker:
Apr
Distributed in:
Salmonella enterica subsp. enterica TA1535 (Salmonella typhimurium)
Cultivation conditions: TGA-MEDIUM . 37° C
TGA-MEDIUM:
Tryptone (Bacto) 10.0 g Glucose 2.0 g NaCl 5.0 g Distilled water 1000.0 ml Adjust pH to 7.0.
The SOS/umu assay is used to evaluate the ability of testing substance or sample to induce DNA damage. The system is
based on alterations in the induction of SOS response as a consequence of DNA damage. SOS/umu assay is convenient
as a screening assay for large number of samples as it is a fast and simple method. The test is standardised for the
determination of the genotoxic potential of water and wastewater. (ISO/DIS 13829, 2000. Determination of genotoxicity of
water and wastewater using the umu-test. TC 147/SC 5.)
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