pENTR 11 DualGateway系統(tǒng)載體質(zhì)粒圖譜序列抗性價(jià)格報(bào)價(jià)Biovector NTCC Inc.
- 價(jià) 格：￥1920
- 貨 號(hào)：BiovectorpENTR 11 Dual
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作微信:1843439339 (QQ同號(hào))
手機(jī)：18901268599
地址：北京
- 已注冊(cè)
pENTR11Dual載體基本信息
| 出品公司: | Invitrigen |
|---|---|
| 載體名稱: | pENTR11 Dual selection vector, pENTR 11 Dual |
| 質(zhì)粒類型: | Gateway系統(tǒng)載體；入門載體 |
| 高拷貝/低拷貝: | -- |
| 啟動(dòng)子: | -- |
| 克隆方法: | 未知 |
| 載體大小: | 3871 bp |
| 5' 測(cè)序引物及序列: | -- |
| 3' 測(cè)序引物及序列: | -- |
| 載體標(biāo)簽: | -- |
| 載體抗性: | 卡那霉素 （Kanamycin） |
| 篩選標(biāo)記: | 氯霉素 |
| 備注: | 克隆宿主E.coli DB3.1 ，使用方法與pENTR 1A 相同，二者區(qū)別在pENTR 11在多克隆位點(diǎn)處含有于NspV ，而不是DraI。 |
| 產(chǎn)品目錄號(hào): | #A10467 |
| 穩(wěn)定性: | -- |
| 組成型: | -- |
| 病毒/非病毒: | -- |
pENTR11Dual載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
pENTR11Dual載體簡(jiǎn)介
Gateway? pENTR? 11 Dual Selection Vector Gateway? entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway? entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR? Dual Selection vectors! The Gateway? entry vectors (Table 1) offer the following: ? attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway? destination vector to ensure cloning of the gene of interest in the correct orientation for expression? Kozak consensus sequence for efficient translation initiation in eukaryotic systems? Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR? 1A Dual Selection, pENTR?3C Dual Selection, and pENTR?11 Dual Selection vectors only)? rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli ? pUC origin for high-copy replication and maintenance of the plasmid in E. coli? Kanamycin resistance gene for selection in E. coli? The ccdB?chloramphenicol fusion gene located between the two attL sites for negative selection and Chloramphenicol selection in E. coli.? Kanamycin resistance gene for selection in E. colipENTR11Dual載體序列
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCATGGATCTCGGGGACGTCTAA CTACTAAGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTT TCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAC GTTGTGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAACTAAGC AGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTTAGTTAGTTACTTAAGCT CGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGC TTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTTCGAAGGAGATAGAACCAATTCTCTAAGGAAAT ACTTAACCATGGTCGACTGGATCCGGTACCGAATTCGTCGACCTGCAGACTGGCTGTGTATAAGGGAGCC TGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCA GCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCAT CCCCGATATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGG GATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTC TCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTC ATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGAC GACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAAC TGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCG GGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCT TTTAGTAAGCCGGATCCTCTAGATTACGCCCCGCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAG CATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTG TCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTA AATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGG GAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCG TCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAA CACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCG GGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTA ATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTAC GATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCC TGAAAATCTCGACGGATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCT GGGGTGCCTAATGCGGCCGCGAATTCGCGGCCGCACTCGAGATATCTAGACCCAGCTTTCTTGTACAAAG TTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAAT CATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAA AAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCAT ATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGG CTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTT GTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTG ACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCA CTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGA TGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGC GTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACG AGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTC AGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATT GATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGT TTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCA GTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACATTATTCAGATTGG GCCCCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGC GCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCT ACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAA CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC CGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTA TAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT T
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