pP2-sec plasmid短芽孢桿菌分泌型表達載體質粒

P2 Promoter Uses part of the 5' sequence of the cell-wall protein gene.
Has almost no activity in E. coli.
Very strong expression inBrevibacillus.
Sec Signal A modified secretion signal to increase versatility
Multiple Cloning Site 11 restriction enzyme cutting sites
X-Terminator A 46-bp terminator structure downstream from the multiple
cloning site
Rep Gene relating to plasmid replication (from pUB110)
Ori Replication origin for replication of the plasmid inBrevibacillus
(from pUB110)
Nmr Neomycin resistance gene
Selection marker inBrevibacillus
ColE1Ori Origin of replication for the plasmid inE. coli (pUC derived)
Ampr Ampicillin resistance gene
Selection marker inE. coli

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