Brevibacillus Expression System Ⅱ短芽孢桿菌表達(dá)系統(tǒng)

Description
TheBrevibacillus (Bacillus brevis) Expression System is an efficient system for producing high
yields of secreted protein. Using this system, it is possible to produce an unprecedented
number of heterologous proteins. TheBrevibacillus Expression System offers the following
advantages, and is particularly effective in the production of secretory proteins.
?Extracellular secretion of large amounts of protein
?Almost no detectable protease activity
?Produces active proteins
?Simple procedures for genetic manipulation and culturing
?Safe host bacteria
Examples of protein production using this system are shown in Table 1. High expression levels
were obtained for a variety of proteins (enzymes, antigens, and cytokines), regardless of gene
origin (bacteria, archaea and eukaryotes). In particular, secretory proteins of eukaryotic origin
typically contain S-S bonds for biological activity, and it is generally difficult to produce these
proteins using other prokaryotic expression systems. The characteristic secretory production
ofBrevibacillus allows high-efficiency production, even for proteins with S-S bonds.

It is possible to construct the expression vector using a shuttle vector inE. coli or by directly
introducing the DNA ligation solution into the expression host. Target protein may be obtained
by culturing transformed host bacteria in test tubes or flasks (two types of media can be
used; see Section VI. 9 for medium composition), and collecting protein from the culture
supernatant. Homogenization of bacteria is unnecessary; the target protein is obtained from
the clear supernatant fraction following centrifugation (to pellet the bacterial cells) and may
be purified using standard techniques.

Components
Brevibacillus Expression System II (Cat. #HB200)
● Expression Vectors
pNY326 DNA 10 μg (Cat. #HB111)
pNCMO2 DNA 10 μg (Cat. #HB112)
● Control Vector
pNY326-BLA DNA 1 μg (Cat. #HB114)
● Competent Cells
Brevibacillus Competent Cells (Cat. #HB116)
?Brevibacillus Competent Cells 100 μl x 10
?MT medium 1 ml x 10
?Solution A 1 ml
?Solution B 1 ml x 2

Supplier來(lái)源:BioVector NTCC Inc.
TEL電話:400-800-2947
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