pEF1HTLV-Luc plasmid哺乳動物長效熒光強表達載體,適合活體熒光檢測

適合活體熒光檢測
pEF1HTLV-Luc plasmids contain genes that have been chemically
synthesized. The DNA sequence of these genes was modified by
optimizing the codon usage, reducing or eliminating the CpG motifs and
avoiding secondary DNA structures without changing the amino acid
sequence of the wild type proteins.
pEF1HTLV-Luc plasmids may be used:
to subclone the synthetic gene into another vector. To facilitate
subcloning, the Luc gene is flanked by two unique restriction sites:
Nco I at the 5’ end that encompasses the Start codon, and Nhe I at the
3’end.
As a gene reporter plasmid. pSelect-zeo is a mammalian expression
plasmid selectable in E. coli and mammalian cells with Zeocin?, as the
Sh ble gene in the second expression casssette is driven by the eukaryote
CMV enhancer/promoter in tandem with the bacterial EM7 promoter.


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