pKD46-Cas9 plasmid原核基因編輯RED重組質(zhì)粒載體

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Vector typeBacterial Expression, CRISPR ; Lambda Red recombinase
Bacterial Resistance(s)Kanamycin
Growth Temperature30°C
Growth Strain(s)DH5alpha
Growth instructionsMust be grown at 30C! Vector has temperature-sensitive replication (RepA101ts)
Copy numberLow Copy
GENE/INSERT
Gene/Insert namecas9
SpeciesS. pyogenes MGAS5005
Insert Size (bp)4500
Promoternative cas9 promoter

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