Quantitative Synthetic Monkeypox virus DNA猴痘病毒核酸DNA

Quantitative Synthetic Monkeypox virus DNA

This  can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions.

Product category
Viruses
Product type
Molecular standard
Nucleic acid
Organism
Monkeypox virus, MPXV
Applications
Infectious disease research
Assay development
Zoonotic disease research
Specification range
≥ 1 x 105 to 1 x 106 copies/μL
Volume
100 μL
Product format
Frozen
Shipping information
Shipped in a proprietary stabilization matrix

Storage conditions
-20°C or colder

Characteristics
Genetic target
Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions.
Comments
Manufactured under ISO 13485 guidance
Handling information
Handling procedure
Thaw the vial on ice. Avoid exposing the synthetic DNA to repeated freeze-thaw cycles as it may result in degradation of the DNA and variation in copy number.
Gently mix the sample to ensure an even distribution of material.
Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws, which can damage the synthetic DNA. This construct is synthetically derived and therefore does not contain any viable material and cannot replicate.

The following primers and probe can be used with this nucleic acid preparation.
Forward primer (5’ to 3’): CATCTATTATAGCATCAGCATCAGA
Reverse primer (5’ to 3’): GATACTCCTCCTCGTTGGTCTAC
Probe (5’ to 3’): JOE/TGTAGGCCGTGTATCAGCATCCATT/BHQ1

Quality control specifications
Quality accreditation
Manufactured under ISO 13485 guidance

Supplier來源:BioVector NTCC Inc.
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