pCSN074酵母基因編輯質(zhì)粒

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Vector backbone pRS414

Backbone size w/o insert (bp)7206
Total vector size (bp)10926
Vector typeYeast Expression, CRISPR
Selectable markersTRP1 ; KanMX
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberUnknown
GENE/INSERT
Gene/Insert namedCas12a-NLS
Alt namedCpf1-NLS
SpeciesSynthetic; Lachnospiraceae bacterium ND2006
Insert Size (bp)3720
MutationE925A
PromoterS. cerevisiae TEF1
Tag / Fusion Protein
SV40 NLS (C terminal on insert)
CLONING INFORMATION
Cloning methodUnknown
5′ sequencing primerATGTCTAAGTTGGAAAAATTCACCA
3′ sequencing primerTTATACCTTTCTCTTCTTCTTTGG

Supplier來源:BioVector NTCC Inc.
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